Stable to expiry when stored at 15-25°C
Completely automated protocols
Are available for a wide range of analysers
Serum iron 143 µmol/l, UIBC 89.4 µmol/l
- Colorimetric method
- Lyophilised reagents
- Linearity: Serum iron 143 µmol/l, UIBC 89.4 µmol/l
- Stable to expiry when stored at 15-25°C
What is Iron/UIBC assay used for?
In the measurement of serum iron, ferric iron is dissociated from its carrier protein, transferrin, in an acid medium and simultaneously reduced to the ferrous form. The ferrous iron is then complexed with the chromogen, a sensitive iron indicator, to produce a blue chromophore which absorbs at 595 nm.
In the measurement of unsaturated iron binding capacity (UIBC) a known amount of ferrous iron is added, in excess, to the serum at alkaline pH. This saturates the unoccupied iron binding sites on the transferrin. The amount of free iron thus measured is subtracted from the total amount added to calculate the UIBC.