A Myositis Biomarker
Benefits of the Randox Aldolase Assay
A correlation coefficient of r=0.9917 was displayed when the Randox methodology was compared against commercially available methods.
Excellent measuring range
The Randox aldolase assay has a measuring range of 1.73 – 106U/l for the comfortable detection of clinically important results.
Lyophilised reagents offer enhanced stability, reducing wastage.
The Randox assay displayed a within run precision < 4.47% CV.
Dedicated calibrator and controls available
Randox offer a dedicated aldolase calibrator and controls for a complete testing package.
Applications available detailing instrument-specific settings for the convenient use of the Randox aldolase assay on a variety of clinical chemistry analysers.
Aldolase is a glycolytic enzyme responsible for catalysing the conversion of fructose 1-6-diphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate via the glycolysis metabolic pathway. This enzyme is present in all bodily cells, more commonly in the nucleus and cytoplasm. It has been identified as having three isoforms: A, B and C. Isoform A is found bound to the actin-containing filament of the cytoskeleton. By binding (reversible) to these filaments, aldolase aids in regulating cell contractions. The highest concentrations of this enzyme are present in the brain, liver and muscles 1.
Elevated levels of aldolase are detected in myotonic muscular disease, including: polymyositis and progressive muscular dystrophy. Elevated levels of this enzyme have been observed in acute coronary syndromes 1. This enzyme has been identified as a myositis biomarker, a muscle-wasting disease resulting in reduced muscle strength and fatigue 2. Testing this enzyme can be utilised as a marker in the differential diagnosis of muscle weakness as aldolase levels remain consistent where weakness is caused by neurological problems such as multiple sclerosis (MS) 3.
 Berridge BR, Van Vleet JF, Herman E. Cardiac, Vascular, and Skeletal Muscle Systems. Haschek WM, Rousseaux CG, Wallig MA (eds). Haschek and Rousseaux’s Handbook of Toxicologic Pathology, 3rd ed. Academic Press; 2013. pp. 1567-1665
 The Myositis Association. About Myositis. https://www.myositis.org/about-myositis/ (accessed 27 February 2020)
 The Myositis Association. Blood Tests. https://www.myositis.org/about-myositis/diagnosis/blood-tests/ (accessed 27 February 2020).