Key Benefits of the Randox G-6-PDH Reagent
The Randox G-6-PDH test does not suffer from interferences due to the sample prewash step
Excellent correlation with standard methods
The Randox methodology has been compared against other commercially available methods and the Randox G-6-PDH assay showed a correlation coefficient of r=0.99
The Randox G-6-PDH assay has a reconstituted stability of 4 weeks when stored at +2 – +8⁰C
Other features of the Randox G-6-PDH reagent
- UV method
- Lyophilised reagents
- Reconstituted stability of 4 weeks when stored at +2 – +8⁰C
- Measuring range 154 – 4303 U/l
Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers. Contact us to enquire about your specific analyser.
What is the G-6-PDH assay used for?
What is G-6-PDH?
Glucose-6-phosphate dehydrogenase (G-6-PDH) is a cytosolic enzyme located on the X-chromosome and can be found in every bodily cell. Glucose-6-phosphate dehydrogenase is involved in the normal processing of carbohydrates. G-6-PDH plays a critical role in red blood cells (RBC’s), protecting them from damage and premature destruction. The two main products of G-6-PDH are ribose-5-phosphate which is important for DNA, the chemical cousin of RNA. The chemical reaction produces NADPH which protects bodily cells from reactive oxygen species.
What is the G-6-PDH assay used for?
Glucose-6-phosphate dehydrogenase (G-6-PDH) deficiency is the most common enzyme deficiency in the pentose phosphate pathway, affecting more than 400 million people globally. G-6-PDH deficiency is an X-linked recessive disorder mainly affecting red blood cells (RBC’s).
A defect in the G-6-PDH enzyme results in premature haemolysis (break down of RBC’s). If the bone marrow cannot compensate for the reduction of RBC’s, haemolytic anaemia can occur. Many individuals that are glucose-6-phosphate dehydrogenase deficient are asymptomatic most of the time, however when they are exposed to certain triggering factors, they can develop acute haemolytic anaemia (AHA), which can be life-threatening, especially in children. Symptoms associated with G-6-PDH deficiency can include paleness, jaundice, dark urine, fatigue, shortness of breath, a sudden rise in body temperature, lower back pain, splenomegaly (enlarged spleen) and a rapid heart rate. Other symptoms can include nausea, diarrhoea or abdominal discomfort. It has been noted that glucose-6-phosphate dehydrogenase deficiency is a significant cause of mild to severe jaundice in new-borns. For more information on haemolytic anaemia, please click here [external link]. Early and accurate diagnosis of glucose-6-phosphate dehydrogenase deficiency is essential in ensuring the successful management of haemolytic anaemia.
The Randox G-6-PDH assay is used for the quantitative in vitro determination of glucose-6-phosphate dehydrogenase in erythrocytes. The enzyme activity is determined by measurement of the rate of absorbance change at 340 nm due to the reduction of NADP+.
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