Reagent | HDL Cholesterol

Key Benefits

There are several methods available for measurement of HDL Cholesterol

Including Direct Clearance and Phosphotungstic Acid

Exceptional correlation with standard methods

A correlation coefficient of r=0.99 for the Precipitation and Ultracentrifugation methods, was verified against the CDC reference method

Excellent stability

Stable to expiry date when stored at +2 to +8ºC

HDL Cholesterol-Direct Clearance Method

  • Direct Clearance Method
  • Liquid ready-to-use reagents
  • Stable to expiry when stored at 2-8°C
Cat NoSize
CH1383R1 3 x 2.5L (L)
R2 1 x 2.5L
EnquireKit Insert RequestMSDSBuy Online
CH2655R1 6 x 78ml (L)
R2 3 x 52ml
EnquireKit Insert RequestMSDSBuy Online
CH3811R1 3 x 51ml (L)
R2 3 x 20ml
EnquireKit Insert RequestMSDSBuy Online
CH8033R1 4 x 38.2ml (L)
R2 4 x 18.2ml
EnquireKit Insert RequestMSDSBuy Online
CH8311R1 4 x 20ml (L)
R2 4 x 9ml
EnquireKit Insert RequestMSDSBuy Online
(L) Indicates liquid option

Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers.  Contact us to enquire about your specific analyser.

Randox HDL Cholesterol Precipitant-Phosphotungstic Acid

  • AMP method
  • Liquid and lyophilised reagents available
  • Stable to expiry when stored at +15 to +25°C
  • Wide measuring range of 0.071-23.7 mmol/l
Cat NoSize
CH203 *4 x 80ml (L)EnquireKit Insert RequestMSDSBuy Online
* CH203 is a precipitant product which can be used alongside the CHOD PAP assays
(L) Indicates liquid option

Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers.  Contact us to enquire about your specific analyser.

What is the HDL Cholesterol assay used for?

High-density lipoproteins (HDL) are one of the major classes of plasma lipoproteins. They are composed of a number of heterogeneous particles, including cholesterol and vary with respect to size and content of lipid and Apolipoprotein. HDL serves to remove cholesterol from the peripheral cells to the liver, where the cholesterol is converted to bile acids and excreted into the intestine.

Accurate measurement of HDL-C is of vital importance when assessing patient risk from CHD. Direct measurement gives improved accuracy and reproducibility when compared to precipitation methods.

HDL is usually requested with other tests, either with cholesterol or as part of a lipid profile, including LDL and triglycerides. The combination of total cholesterol and HDL is very useful for screening for the risk of heart disease.

Patients at risk include:

    • Smokers
    • Men over 45 years and women over 55 years
    • Those with Hypertension (blood pressure 140/90 or higher or if taking medications for high blood pressure)


    • Wallace, J.M.W., et al. Boron supplementation and activated factor VII in healthy men. EJCN 2002, 56(11): 1102-1107
    • Joshi, S., et al. Fish oil supplementation of rats during pregnancy reduces adult disease in their offspring. J. Nutr., 2003, 133: 3170-3174
    • Ghorbanihaghjo, A., et al. Effect of nandrolone decanoate on serum lipoprotein (a) and its isoforms in hemodialysis patients. Lipids Health Dis. 2004, 3: 16
    • Saeed, B.O., et al. Comparison of three homogeneous methods for measuring high-density lipoprotein cholesterol with a precipitation method in diabetic and non-diabetic subjects. Br. J. Biomed. Sci., 2004, 61(4): 194-199
    • Nowak, M., et al. Changes in lipid metabolism in women with age-related macular degeneration. Clin. Exp. Med., 2005, 4(4): 183-187
    • Moreira Lima, L., et al. Níveis Plasmàticos Elevados de Lipoproteína(a) Correlacionados com a Gravidade da Doenca Arterial Coronariana em Pacientes Submetidos à Angiografia (Increased Serum Levels of Lipoprotein(a) Correlated with the Severity of Coronary Artery Disease in Patients Submitted to Angiography. Arquivos Brasileiros de Cardiologia. 2006, 87(3): 260-266
    • Mineo, D., et al. Effects of lung volume reduction surgery for emphysema on glycolipidic hormones. Chest. 2008, 134(1): 30-37
    • Bajaj, S., et al. A case-control study on insulin resistance, metabolic co-variates and prediction score in non-alcoholic fatty liver disease. Indian J. Med. Res. 2009, 129: 285-292
    • Hossein-nezhad, A., et al. Association of VDR gene polymorphism with insulin resistance in diabetic patients. Iranian Journal of Diabetes and Lipid Disorders. 2009, 143-150
    • Komolafe, O.A., et al. Streptozotocin-induced diabetes alters the serum lipid profiles of adult Wistar rats. The internet Journal of Cardiovascular Research 2009, 7(1) doi: 10.5580/2251
    • Lim, W,Y.A., et al. Lipoprotein lipase expression, serum lipid and tissue lipid deposition in orally administered glycyrrhizic acid-treated rats. Lipids in Health and Disease, 2009, 8: 31
    • Rhodes, P., et al. Adult-onset obesity reveals prenatal programming of glucose-insulin sensitivity in male sheep nutrient restricted during late gestation. PloS ONE 2009, 4(10): e7393
    • Velasco-Martínez, R.M., et al. Obesity and insulin resistance among adolescents from Chiapas. Nutr. Hosp. 2009, 24(2)
    • Eu, C.H.A., et al. Glycyrrhizic acid improved lipoprotein lipase expression, insulin sensitivity, serum lipid and lipid deposition in high-fat diet-induced obese rats. Lipids and Health Disease. 2010, 9: 81
    • Mahadik, S.R. et al. Role of adipocytokines in insulin resistance: Studies from Urban Western Indian Population. Int. J. Diabetes & Metab. 2010, 18(9): 35-42
    • Wonnacott, K.E. et al. Dietary omega-3 and-6 polyunsaturated fatty acids affect the composition and development of sheep granulose cells, oocytes and embryos. Reproduction. 2010, 139(1): 57-69
    • Ganguli, D., et al. Association between inflammatory markers and cardiovascular risk factors in women from Kolkata, W.B, India. Arq. Bras. Cardiol. 2011, 96(1): Epub
    • Kojic, Z., et al. Effect of captopril on serum lipid levels and cardiac mitochondrial oxygen consumption in experimentally-induced hypercholesterolemia in rabbits. Physiol. Res. 2011, 60(1): S177-S184
    • Srinivasa, G., et al. Comparison between serum insulin levels and its resistance with biochemical, clinical and anthropometric parameters in South Indian children and adolescents. Ind. J. Biochem. 2011, 26(1): 22-27
    • Hossein-nezhad, A. et al. Circulating omentin-1 in obesity and metabolic syndrome status compared to control subjects. Endocrinol. Metabol. Syndrome 2012: S1:008 doi:10.4172/2161-1017.S1-008
    • Yahaya, N. et al. Type 2 diabetes with good glycemic control have improved insulin response and lower non-esterified fatty acid level after a meal challenge. Journal of Diabetes Mellitus 2012, 2(1): 1-7