Non-Esterified Fatty Acids (NEFA) Assay

Home - Non-essential Fatty Acids Assay

Non-Esterified Fatty Acids (NEFA) Assay

Reagent | Non-Esterified Fatty Acid (NEFA)

A Marker of Insulin Resistance

Benefits of the Randox NEFA Assay

Correlation

Exceptional correlation

A correlation coefficient of r=0.98 was displayed when the Randox NEFA assay was compared to commercially available methods.

Precision

Excellent precision

The Randox NEFA assay displayed a precision of <5% CV.

Extensive measuring range

The Randox NEFA assay has a measuring range of 0.072 – 2.24mmol/l for the comfortable detection of clinically important results.

Standard or Calibrator

Standard supplied with the kit

The Randox NEFA kit includes the standard simplifying the ordering process.

Controls available

Controls available offering a complete testing package.

Applications available

Applications available detailing instrument-specific settings for the convenient use of the Randox NEFA assay on a variety of clinical chemistry analsyers.

  • Ordering Information
  • PHYSIOLOGICAL SIGNIFICANCE
  • Clinical Significance
Cat NoSize    
FA115R1 3 x 10ml (C)
R2 3 x 20ml
EnquireKit Insert RequestMSDSBuy Online
(C) Indicates calibrator included in kit

Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers.  Contact us to enquire about your specific analyser.

Non-esterified fatty acids are important metabolites stored in adipose tissue. NEFA turnover is swift, with a plasma half – life of 2 to 4 minutes. The dominant source of NEFA is abdominal subcutaneous fat, with considerably less found in leg adipose tissue and a small proportion found in the intraabdominal adipose tissue. NEFA has been recognised as a vehicle by which triacylglycerol (TG) (stored in the adipose tissue) is transported to its sites of utilisation 1. NEFA has been identified as the major source for skeletal muscle during fasting stages and long periods between meals. Cross – sectional studies have consistently documented that circulating NEFA levels are proportional to body fat storage and demonstrated positive correlations between fasting NEFA levels and obesity, insulin resistance and glucose tolerance 2.

Non-esterified fatty acids concentrations are strongly associated with insulin resistance. In the fasting state, the resistance of adipose tissue to the antilipolytic effect of insulin causes the extensive release of NEFA into circulation. Consequently, elevated NEFA levels exacerbate insulin resistance through diminishing insulin – stimulated glucose intake into the skeletal muscle, directly affecting insulin signalling 3.

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