Total Antioxidant Status (TAS) Assay

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Total Antioxidant Status (TAS) Assay

Reagent | Total Antioxidant Status (TAS) 

A Marker of Overall Antioxidant Status

Benefits of the Randox TAS Assay

Superior method

The Randox TAS assay utilises the colorimetric method, offering convenience and efficiency in comparison to ELISA technology and produces results in as little as 3 minutes.

Excellent measuring range

The Randox TAS assay has a measuring range of 0.21 – 2.98mmol/l enabling the comfortable detection of clinically important results.

Lyophilised reagents

Lyophilised reagents offer enhanced stability, reducing wastage.

Standard supplied with the kit

The standard is supplied with the TAS kit, simplifying the ordering process.

Dedicated TAS control available

Dedicated TAS control available offering a complete testing package.

Applications available

Applications available detailing instrument-specific settings for the convenient use of the Randox TAS assay on a variety of clinical chemistry analsyers.

Ordering information

  • Ordering Information
Cat NoSize    
NX23325 x 10ml (S)EnquireKit Insert RequestMSDSBuy Online
(S) Indicates standard included in kit

Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers.  Contact us to enquire about your specific analyser.

  • PHYSIOLOGICAL SIGNIFICANCE
  • Clinical Significance

Free radicals / reactive oxygen species (ROS) are produced during normal cellular metabolism, however, can have harmful effects. Antioxidants are the first line of defence and are produced by the body to neutralise the harmful effects of ROS, preventing cellular damage 1,2, 3. Measuring total antioxidant status (TAS) can provide information on an individual’s overall antioxidant status, which may include antioxidants not yet recognised or not easily measured. The TAS of a sample is a quantitative measurement of the state of balance of the various components (exerting actions in different way) under specified reaction conditions 4.

Reduced levels of total antioxidant status (TAS) is indicative of oxidative stress and increased susceptibility to oxidative damage 5. Oxidative stress is an imbalance between the ROS and antioxidants in the body, favouring ROS 6. Oxidative stress is associated with several health conditions, including: chronic inflammation, neurodegenerative diseases such as Alzheimer’s disease and Parkinson’s disease, cancer and CVD 7.

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Glutathione Peroxidase (Ransel)

Reagent | Glutathione Peroxidase (Ransel)

A Marker of Oxidative Stress

Key Benefits

Superior Performance

Superior method

The Randox Ransel assay utilises the enzymatic method enabling the sensitive and accurate detection of glutathione peroxidase.

Precision

Excellent precision

The Randox Ransel assay displayed a within run precision of <4.86% CV.

Correlation

Excellent correlation

A correlation coefficient of r=0.9829 was displayed when the Randox Ransel assay was compared to commercially available methods.

Measuring range

Excellent measuring range

The Randox Ransel has a measuring range of 75 – 925U/l for the comfortable detection of clinically important results.

Calibrator & Controls

Dedicated calibrator and control available

Dedicated Ransel calibrator and control available offering a complete testing package.

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Applications available

Applications available detailing instrument-specific settings for the convenient use of the Randox Ransel assay on a variety of clinical chemistry analysers.

Ordering Information

Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers.  Contact us to enquire about your specific analyser.

Diagnostic Uses

  • PHYSIOLOGICAL SIGNIFICANCE
  • Clinical Significance

Glutathione peroxidase (GPx) is a reactive oxygen species (ROS), generated by bodily cells via mitochondrial and enzymatic sources. It is vital that GPx levels are reviewed as it can cause oxidative damage to membrane lipids, proteins and DNA. GPx is an intracellular antioxidant enzyme that enzymatically converts hydrogen peroxide to water (detoxification). Hydrogen peroxidase is vital for the maintenance of normal thiol redox-balance, mitochondrial function, and growth factor-mediated signal transduction. By converting hydrogen peroxidase to water, GPx aids in modulating these processes 1. GPx also contributes to cell signalling and oxidative protein folding 2.

Four of the eight glutathione peroxidases (GPx) are selenoproteins (containing the amino acid form of selenium, Sec) in humans, however, GPx6 is cysteine-containing in rodents, but a selenoprotein in humans, whereas the remaining GPx’s are cysteine containing. Glutathione peroxidase 4 (GPx4) is emerging as one of the most important seleoproteins in mammals and is one of the key regulators of ferroptosis, a form of regulated necrotic cell death 2.

GPx displays an inverse correlation with free radicals and consequently oxidative stress (OS). When GPx activity is reduced, antioxidant protection is impaired, resulting in oxidative damage to the membrane fatty acids and functional proteins, resulting in neurotoxic damage. Decreased GPx activity and OS is implicated in the incidence and progression of several health problems, including: cardiovascular disease (CVD), diabetes, atherosclerosis, neurodegenerative disorders and other chronic conditions 3.

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Non-Esterified Fatty Acids (NEFA) Assay

Reagent | Non-Esterified Fatty Acid (NEFA)

Non-Esterified Fatty Acid (NEFA): A Marker of Insulin Resistance

Benefits of the Randox NEFA Assay

Exceptional correlation

A correlation coefficient of r=0.98 was displayed when the Randox NEFA assay was compared to commercially available methods.

Applications available

Applications available detailing instrument-specific settings for the convenient use of the Randox NEFA assay on a variety of clinical chemistry analsyers.

Extensive measuring range

The Randox NEFA assay has a measuring range of 0.072 – 2.24mmol/l for the comfortable detection of clinically important results.

Standard supplied with the kit

The Randox NEFA kit includes the standard simplifying the ordering process.

Controls available

Controls available offering a complete testing package.

Excellent precision

The Randox NEFA assay displayed a precision of <5% CV.

Ordering information

  • Ordering Information
Cat NoSize    
FA115R1 3 x 10ml (C)
R2 3 x 20ml
EnquireKit Insert RequestMSDSBuy Online
(C) Indicates calibrator included in kit

Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers.  Contact us to enquire about your specific analyser.

  • PHYSIOLOGICAL SIGNIFICANCE
  • Clinical Significance

Non-esterified fatty acids are important metabolites stored in adipose tissue. NEFA turnover is swift, with a plasma half – life of 2 to 4 minutes. The dominant source of NEFA is abdominal subcutaneous fat, with considerably less found in leg adipose tissue and a small proportion found in the intraabdominal adipose tissue. NEFA has been recognised as a vehicle by which triacylglycerol (TG) (stored in the adipose tissue) is transported to its sites of utilisation 1. NEFA has been identified as the major source for skeletal muscle during fasting stages and long periods between meals. Cross – sectional studies have consistently documented that circulating NEFA levels are proportional to body fat storage and demonstrated positive correlations between fasting NEFA levels and obesity, insulin resistance and glucose tolerance 2.

Non-esterified fatty acids concentrations are strongly associated with insulin resistance. In the fasting state, the resistance of adipose tissue to the antilipolytic effect of insulin causes the extensive release of NEFA into circulation. Consequently, elevated NEFA levels exacerbate insulin resistance through diminishing insulin – stimulated glucose intake into the skeletal muscle, directly affecting insulin signalling 3.

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A-Z Reagents


Urinary Protein

Reagent | Urinary Protein

Key Benefits

Applications available

For a wide variety of clinical chemistry analysers including the RX series

Strong correlation

The Urinary Protein assay showed a correlation coefficient of 0.9998 against another commercially available method

Standard included in kit

Simplifying the ordering process

Randox Urinary Protein (Colorimetric)

  • Colorimetric method
  • Liquid ready-to-use reagents
  • Stable to expiry date when stored at 15-25⁰C
  • Measuring range 0.022 – 2.14 g/l
  • Applications available
Cat NoSize    
UP15703 x 100ml (S)EnquireKit Insert RequestMSDSBuy Online
UP81134 x 20ml (S)EnquireKit Insert RequestMSDSBuy Online
(S) Indicates standard included in kit

Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers.  Contact us to enquire about your specific analyser.

What is Urinary Protein assay used for?

Determination of Total Protein in urine and cerebrospinal fluid is valuable in the diagnosis of renal and central nervous system disorders respectively. Urinary protein elevations are commonly seen in the following conditions: strenuous exercise, fever and hypothermia, nephrosis and diabetic nephropathy and urinary tract infections. Determination of total protein in cerebrospinal fluid aids in the diagnosis of such conditions as meningitis, CNS tumours and cerebral haemorrhage.

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Uric Acid

Reagent | Uric Acid

Key Benefits

Applications available

For a wide variety of clinical chemistry analysers including the RX series

Strong correlation

The Uric Acid assay showed a correlation coefficient of 0.99 against another commercially available method

Excellent stability

The Uric Acid assay has a precision of less than 4% CV

Randox Uric Acid

  • Enzymatic Colorimetric method
  • Liquid and lyophilised reagents available
  • Stable to expiry date when stored unopened protected from light
  • Measuring range 20.8-1376 µmol/l in serum
  • Applications available
Cat NoSize    
UA2306 x 15ml (S)EnquireKit Insert RequestMSDSBuy Online
UA3824R1 6 x 51ml (L)
R2 4 x 20ml
EnquireKit Insert RequestMSDSBuy Online
UA38709 x 51ml (L)EnquireKit Insert RequestMSDSBuy Online
UA8069R1 6 x 56ml (L)
R2 6 x 20ml
EnquireKit Insert RequestMSDSBuy Online
UA8333R1 4 x 20ml (L)
R2 4 x 7ml
EnquireKit Insert RequestMSDSBuy Online
(L) Indicates liquid option (S) Indicates standard included in kit

Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers.  Contact us to enquire about your specific analyser.

What is Uric Acid assay used for?

Uric acid measurements are used in the diagnosis and treatment of numerous renal and metabolic disorders including renal failure, gout, leukemia and psoriasis. Uric acid is a potent antioxidant contributing to around half the antioxidant capacity of blood plasma. It is a scavenging antioxidant that acts by inactivating free radicals such as HO and HOCI.

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Urea

Reagents | Urea

Key Benefits

Applications available

For a wide variety of clinical chemistry analysers including the RX series

Strong correlation

The Urea assay showed a correlation coefficient of 0.9935 against another commercially available method

Liquid and lyophilised reagents available

Offering convenience and choice

Randox Urea (Kinetic)

  • Kinetic method
  • Liquid and lyophilised reagents available
  • Stable to expiry when stored at 2-8oC
  • Measuring range 0.866 – 56.7 mmol/l in serum and plasma
  • Applications available
Cat NoSize    
UR446
10 x 50ml (S)
1 minute read
EnquireKit Insert RequestMSDSBuy Online
(L) Indicates liquid option (S) Indicates standard included in kit

Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers.  Contact us to enquire about your specific analyser.

Randox Urea (Berthelot)

  • Berthelot method
  • Liquid ready-to-use reagents
  • Working reagent stable for 1 month at 2-8oC
  • Measuring range 1.59-40 mmol/l in serum and plasma
  • Applications available
Cat NoSize    
UR1068170ml (S)
Manual use only
EnquireKit Insert RequestMSDSBuy Online
(S) Indicates standard included in kit

Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers.  Contact us to enquire about your specific analyser.

Randox Urea (Enzymatic Kinetic)

  • Enzymatic Kinetic method
  • Liquid ready-to-use reagents
  • Stable to expiry when stored at 2-8oC
  • Measuring range 0.510 – 50.5 mmol/l in serum and plasma
  • Applications available
Cat NoSize    
UR3825R1 6 x 51ml
R2 4 x 20ml
EnquireKit Insert RequestMSDSBuy Online
UR9781R1 6 x 68ml
R2 6 x 44ml
EnquireKit Insert RequestMSDSBuy Online
UR3873R1 6 x 17.5ml (L)
R2 6 x 3.5ml
EnquireKit Insert RequestMSDSBuy Online
UR8334R1 4 x 20ml (L)
R2 4 x 5.5ml
EnquireKit Insert RequestMSDSBuy Online
UR8070R1 6 x 56ml (L)
R2 6 x 20ml
EnquireKit Insert RequestMSDSBuy Online
(L) Indicates liquid option

Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers.  Contact us to enquire about your specific analyser.

What is Urea assay used for?

Urea is synthesized in the liver from ammonia, as a result of deamination of amino acids. This biosynthetic pathway is the chief means of excretion of surplus nitrogen by the body. Measurements obtained by this test are used in the diagnosis of renal and metabolic disorders, most frequently kidney disorders. More than 60% of the kidney must be destroyed before plasma urea levels are significantly raised. This test is most frequently used in conjunction with serum creatinine for increased protein levels (cardiac decompensation, water depletion).

  • Sánchez-Rodríguez, M.A., et al Antioxidant capacity in relationship to serum lipid peroxides levels in healthy elderly of Mexico City. 2004, 38(2): 193-198.
  • Wathes, D.C., et al Differences between primiparous and multiparous dairy cows in the inter-relationships between metabolic traits, milk yield and body condition score in the periparturient period. Domestic Animal Endocrinology. 2007, 33(2): 203-225.
  • Badiou. S., et al. Fine-tuning of the prediction of mortality in hemodialysis patients by use of cytokine proteomic determination.Clin. J. Am. Soc. Nephrol. . 2006, 3: 423-430.
  • Rhodes, P., et al. Adult-onset obesity reveals prenatal programming of glucose-insulin sensitivity in male sheep nutrient restricted during late gestation. PloS ONE 2009, 4(10): e7393.

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Total Protein

Reagent | Total Protein

Key Benefits

Excellent precision

The Total Protein assay has a precision of less than 3% CV

Applications available

For a wide variety of clinical chemistry analysers including the RX series

Exceptional correlation

The Total Protein assay showed a correlation coefficient of 1.00 against another commercially available method

Randox Total Protein (Biuret)

  • Biuret method
  • Liquid reagents
  • Stable to expiry at 15-25⁰C
  • Measuring range 2.45-150 g/l
  • Applications available
Cat NoSize    
TP2452 x 500ml (S)EnquireKit Insert RequestMSDSBuy Online
TP38699 x 51mlEnquireKit Insert RequestMSDSBuy Online
TP4001R1 4 x 51ml
R2 4 x 44ml
EnquireKit Insert RequestMSDSBuy Online
TP8066R1 4 x 68ml
R2 4 x 68ml
EnquireKit Insert RequestMSDSBuy Online
TP8336R1 4 x 20ml
R2 4 x 17ml
EnquireKit Insert RequestMSDSBuy Online
(S) Indicates standard included in kit

Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers.  Contact us to enquire about your specific analyser.

What is Total Protein assay used for?

Total Protein measurements obtained by the Biuret method are used in the diagnosis and treatment of a variety of diseases involving the liver, kidney and bone marrow as well as other metabolic or nutritional disorders.

  • McGrath L.T., et al. Increased oxidative stress in Alzheimer’s disease as assessed with 4-hydroxynonenal but not malondialdehyde. Q J Med. 2001, 94: 485-490
  • Mula-Abed, W-A. S. and Hanna, B.E. Measurement of serum fructosamine as an index of glycated protein in patients with nephrotic syndrome and chronic liver diseases. Bahrain Medical Bulletin 2001, 23(4)
  • Nishina, H., et al. Effect of nutritional restriction in early pregnancy on isolated femoral artery function in mid-gestation fetal sheep. J. Physiol. 2003, 553(2): 637-647
  • Ozdemir, D., et al. Effect of melatonin on brain oxidative damage induced by traumatic brain injury in immature rats. Physiol. Res., 2005, 54(6): 631-637
  • Mula-Abed, W-A. S. and Aziz, S.B. Serum fructosamine (glycated protein) and related biochemical parameters during normal pregnancy. JBMS Journal of the Bahrain Medical Society 2006, 18(3): 115-122
  • Saad, S.Y. et al. Cardioprotective effects of subcutaneous ebselen against daunorubicin-induced cardiomyopathy in rats. Basic Clin. Pharmacol. Toxicol. 2006, 99(6): 412-417
  • Belaïd-Nouira, Y., et al. Study of lipid profile and parieto-temporal lipid peroxidation in AICI3 mediated neurotoxicity. Modulatory effect of fenugreek seeds. Lipids Health Dis. 2012, 11: 16
  • Yakubu, N., et al. Antioxidant and hepatoprotective properties of tofu (curdle soymilk) against acetaminophen-induced live damage rats. Biotech. Res. Int. 2013, ID 230142

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Sodium

Reagent | Sodium

Key Benefits

Exceptional correlation

The Sodium assay showed a correlation of r=0.98 against another commercially available method

Applications available

For a wide variety of clinical chemistry analysers including the RX series

Excellent stability

Working reagent stable for 2 weeks at 2-8°C or 5 days at 15-25°C

Randox Sodium (Enzymatic)

  • Enzymatic method
  • Lyophilised reagents
  • Working reagent stable for 2 weeks at 2-8°C or 5 days at 15-25°C
  • Measuring range 37.3-187.8 mmol/l
Cat NoSize    
NA3851R1 3 x 20ml
R2 3 x 9ml
EnquireKit Insert RequestMSDSBuy Online
NA8327R1 4 x 10ml
R2 4 x 6ml
EnquireKit Insert RequestMSDSBuy Online
(S) Indicates standard included in kit

Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers.  Contact us to enquire about your specific analyser.

What is Sodium assay used for?

Sodium measurements are used in the diagnosis and treatment of aldosteronism, diabetes insipidus, adrenal hyper-tension, Addison’s disease, dehydration, inappropriate antidiuretic hormone secretion, or other diseases involving electrolyte imbalance.

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Ethanol Reagent

Reagent | Ethanol

Key Benefits of Ethanol

Excellent correlation

The Randox ethanol assay displayed an excellent correlation coefficient of r=0.995 when compared against the gold standard Gas Chromatography / Mass Spectrometry (GC/MS).

Dedicated calibrator/control set available

A dedicated ethanol calibrator/control set is a available for a full testing package.

Applications available

Applications are available detailing instrument-specific settings for the convenient use of the Randox ethanol assay on a wide range of clinical chemistry analysers.

Other features of the Ethanol reagent

  • Enzymatic method using NAD and alcohol dehydrogenase
  • Stable to expiry date when stored at +2 to +8°C
  • Wide measuring range of 3 – 500mg/dl
  • Suitable for use with both urine and serum samples

Ordering Information

Cat NoSize    
DA4015R1 2 x 16.9ml
R2 2 x 8ml
EnquireKit Insert RequestMSDSBuy Online

Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers.  Contact us to enquire about your specific analyser.

What is the Ethanol assay used for?

Ethyl alcohol is present in some drinks, liquors, medicinal preparations and foods. After ethyl alcohol ingestion, the alcohol quickly spreads though the whole body and is mostly metabolized in the liver and excreted. Ethyl alcohol acts as a Central Nervous System depressant, and can lead to loss of alertness, stupor, coma and death and frequently cause public safety issues. Alcohol consumption during pregnancy carries a heavy risk of permanent mental and physical defects in the child, known as fetal alcohol syndrome. Ethyl alcohol measurements are also used in diagnosis and treatment of alcohol intoxication and poisoning.

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Fructosamine (Glycated Protein) Assay

Reagent | Fructosamine (Glycated Protein)

Offering Improved Specialty and Reliability

Benefits of the Fructosamine Assay

Superior Performance

Superior method

The Randox enzymatic method offers improved specificity and reliability compared to the conventional NBT-based methods as the enzymatic method does not suffer from non-specific interferences unlike the existing methods which can also be time-consuming and difficult to automate.

14-C glucose icon

Standardisation to the highest level

The Randox dedicated fructosamine calibrator and controls are assigned relative to human serum glycated with 14-C glucose, directly reflecting the nature of the patient sample.

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Excellent correlation

A correlation coefficient of r=0.98 was displayed with the Randox fructosamine assay was compared to commercially available methods.

Wide measuring range

Excellent measuring range

The Randox fructosamine assay has a measuring range of 8.12 – 18033µmol/l for the comfortable detection of clinically important results.

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Liquid ready-to-use

The Randox fructosamine assay is available in a liquid ready-to-use format for convenience and ease-of-use.

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Applications available

Applications available detailing instrument-specific settings for the convenient use of the Randox fructosamine assay on a variety of clinical chemistry analysers.

Ordering Information

Cat NoSize    
FR3133R1 5 x 25ml
R2 5 x 6.3ml
EnquireKit Insert RequestMSDSBuy Online
FR4030R1 4 x 19.8ml
R2 4 x 6.9ml
EnquireKit Insert RequestMSDSBuy Online

Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers.  Contact us to enquire about your specific analyser.

Diagnostic Uses

  • Clinical Significance
  • Physiological Significance
  • Drawbacks of Traditional Diabetes Tests

Fructosamine (glycated protein) has been identified as an early indicator of diabetic control compared to other markers such as HbA1c. RBCs live for approximately 120 days, HbA1c represents the average blood glucose levels for the previous 2 to 3 months. Conversely, fructosamine has a shorter lifespan, of about 14 to 21 days, reflecting average blood glucose levels from the previous 2 to 3 weeks 1.

Fructosamine testing has been identified as being the best for patient care as HbA1c results can be inconclusive for several reasons. Genetic, haematological and disease-related factors negatively impact HbA1c levels, with low levels observed in late stage chronic kidney disease, conditions that shorten the lifespan of erythrocytes such as haemolytic anaemia, and in certain haemoglobinopathies such as sickle cell disease 2.

In gestational diabetes, fructosamine should be tested as HbA1c levels are difficult to interpret as HbA1c integrates glycaemia over the lifespan of the erythrocyte. Therefore, HbA1c is relatively insensitive to short term changes. Consequently, HbA1c testing isn’t suitable in the monitoring of the effects of changes in medication. Fructosamine is a medium-term marker (shorter life span) and is a much more suitable test 2.

In a diabetic patient where blood glucose levels are abnormally elevated, the concentration levels of fructosamine also increase as fructosamine is formed by a non-enzymatic Maillard reaction between glucose and amino acid residues of proteins. During this glycation process, an intermediate labile Schiff base is produced which is converted to a more stable ketoamine (fructosamine) via an Amadori rearrangement 3.

The Fasting Plasma Glucose (FPG) test measures the level of blood sugars which is used to diagnose and monitor diabetes based on insulin function. The main drawback of this test is that a hormone called glucagon, produced in the pancreas, is triggered during prolonged fasting, signalling the liver to release glucose into the bloodstream. In diabetic conditions, either the body is unable to generate enough insulin or cannot appropriately respond to insulin. Consequently, FPG levels remain high 1.

In the1980’s, HbA1c was incorporated into clinical practice as HbA1c levels correlated well with glycaemic control over a 2 to 3-month period. The main drawback of this test is that any condition that reduces the survival rate of erythrocytes such as haemolytic anaemia will falsely lower the HbA1c test results regardless of the assay method utilised 3.

Related Products

Fructosamine Calibrator

Fructosamine Control

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