Lactate Dehydrogenase

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Lactate Dehydrogenase

Reagent | Lactate Dehydrogenase

Key Benefits

Excellent precision

The LD assay showed a precision of less than 4% CV

Exceptional correlation

The assay showed a correlation of r=0.98 against another commercially available method

Liquid and lyophilised reagents available

Offering convenience and choice

Randox Lactate Dehydrogenase L-P (LDH) (NAD)

  • LDH NAD method
  • Liquid and lyophilised reagents available
  • Stable to expiry at 2-8°C
  • Measuring range 8.8 – 635 U/l
Cat NoSize    
LD3842R1 6 x 20ml
R2 3 x 18ml
EnquireKit Insert RequestMSDSBuy Online
LD8052R1 4 x 68ml
R2 4 x 37.2ml
EnquireKit Insert RequestMSDSBuy Online

Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers.  Contact us to enquire about your specific analyser.

Randox Lactate Dehydrogenase P-L (UV)

  • UV method
  • Liquid and lyophilised reagents available
  • Stable to expiry at 2-8°C
  • Measuring range 42.3-1191 U/L
Cat NoSize    
LD40120 x 3mlEnquireKit Insert RequestMSDSBuy Online
LD3818R1 6 x 20ml (L)
R2 3 x 11ml
EnquireKit Insert RequestMSDSBuy Online
LD8051R1 6 x 56ml (L)
R2 6 x 20ml
EnquireKit Insert RequestMSDSBuy Online
LD8322R1 4 x 20ml (L)
R2 4 x 7ml
EnquireKit Insert RequestMSDSBuy Online
(L) Indicates liquid option

Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers.  Contact us to enquire about your specific analyser.

What is Lactate Dehydrogenase assay used for?

Lactate dehydrogenase (LD) catalyses the interconversion of lactic acid and pyruvic acid. The enzyme is composed of 4 peptide chains and exists in 5 isomeric forms. LD is widely distributed throughout the body, and the highest concentrations are found in the liver, heart and skeletal muscle. LD activity is significantly elevated during myocardial infarction. Maximum levels are reached 24 to 48 hours after the onset of chest pain and may remain elevated for 7 to 12 days post infarction. Increases in LD activity are also associated with other pathological conditions including liver disease, progressive muscular dystrophy, megaloblastic and pernicious anaemia.

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Lactate

Reagent | Lactate

Key Benefits

Excellent stability

Working reagent stable for two weeks at 15-25°C

Exceptional correlation

The assay showed a correlation of r=0.99 against another commercially available method

Wide measuring range

The Randox Lactate assay has a wide measuring range of 0.100 – 19.7 mmol/l, capable of detecting abnormal levels in a sample

Other features

  • Colorimetric method
  • Lyophilised reagents
  • Working reagent stable for 2 weeks at 15-25°C
  • Measuring range 0.100 – 19.7 mmol/l

Ordering information

Cat NoSize    
LC238916 x 6ml (S)EnquireKit Insert RequestMSDSBuy Online
LC39804 x 20.5mlEnquireKit Insert RequestMSDSBuy Online
(S) Indicates standard included in kit

Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers.  Contact us to enquire about your specific analyser.

What is Lactate assay used for?

Increased concentration of lactate in the blood is an indicator of anaerobic metabolism i.e. blood flow to the tissues decrease and oxygen delivery is insufficient. In cases of severe oxygen deprivation “Lactic Acidosis” may occur. L-Lactate may therefore be used as an indicator of severity of circulatory failure.

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Iron

Reagent | Iron

Key Benefits

Excellent precision

The Iron (ferene) assay showed a precision of less than 3.5% CV

Exceptional correlation

The assay showed a correlation of r=0.99 against another commercially available method

Liquid and lyophilised reagents available

Offering convenience and choice

Randox Iron- Ferrozine

  • Ferrozine method
  • Liquid ready-to-use reagents
  • Stable to expiry when stored at 15-25⁰C
  • Measuring range 2.0 – 197 µmol/l
Cat NoSize    
SI3821R1 6 x 20ml
R2 3 x 11ml
EnquireKit Insert RequestMSDSBuy Online
SI8049R1 7 x 15.5ml
R2 7 x 6.7ml
EnquireKit Insert RequestMSDSBuy Online
SI8330R1 4 x 20ml
R2 4 x 7ml
EnquireKit Insert RequestMSDSBuy Online

Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers.  Contact us to enquire about your specific analyser.

What is Iron assay used for?

Iron deficiency is one of the most prevalent human disorders. In adults iron deficiency is often symptomatic of chronic blood loss. Iron deficiency results in deficient haem synthesis with the subsequent symptoms of anaemia due to tissue hypoxia. Iron (non-heme) measurements are used in the diagnosis and treatment of diseases such as iron deficiency anaemia, hemochromatosis (a disease associated with widespread deposit in the tissues of two iron-containing pigments, hemosiderin and hemofuscin, and characterized by pigmentation of the skin), and chronic renal disease.

Clinical Chemistry Panel

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Haemoglobin

Reagent | Haemoglobin

Key Benefits

Applications available

For a wide variety of clinical chemistry analysers

Excellent stability

The diluted reagent is stable for at least 6 months at +15°C to +25°C when stored in a tightly closed dark bottle

Excellent linearity

This method is linear up to 21 g/dl

Other Features

  • Colorimetric method
  • Liquid reagents
  • Stable to expiry when stored at 2-8⁰C
  • Excellent linearity up to 21 g/dl
  • No extraction needed with whole blood application
  • For manual use only

Ordering Information

Cat NoSize    
HG15395 x 100mlEnquireKit Insert RequestMSDSBuy Online

Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers.  Contact us to enquire about your specific analyser.

What is Haemoglobin assay used for?

Haemoglobin measurement is part of the full blood count which is requested when a doctor suspects an illness related to above or below normal levels. Haemoglobin levels are also measured:

  • before operations to make sure the patient is fit for surgery
  • to detect and measure the severity of anaemia or polycythaemia
  • to monitor response to treatment
  • to help make decisions about blood transfusions

Related Products

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Glycerol

Reagent | Glycerol

Key Benefits

Applications available

For a wide variety of clinical chemistry analysers

Exceptional correlation

The Glycerol assay showed a correlation of r=0.999 against another commercially available method

Limited interference

Ascorbic Acid up to a concentration of 400 µmol/l will not affect this test

Other Features

  • GPO-PAP method
  • Lyophilised reagents
  • Working reagent stable for 14 days when stored at 2-8⁰C or 3 days at 15-25°C
  • Measuring range 14.5 – 2545 µmol/l
  • For manual or semi-automated use only
Cat NoSize    
GY1056x 15ml (S)EnquireKit Insert RequestMSDSBuy Online
(S) Indicates standard included in kit

Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers.  Contact us to enquire about your specific analyser.

What is Glycerol assay used for?

Glycerol measurements are used in the diagnosis and treatment of patients with diabetes mellitus, nephrosis, liver obstruction, other diseases involving lipid metabolism, or various endocrine disorders.

  • Beatty, O.L. Insulin resistance in offspring of hypertensive parents. BMJ 1993, 307(6896): 92-96
  • Ranganath, L., et al. The effect of circulating non-esterified fatty acids on the entero-insular axis. European Journal of Clinical Investigation. 1999, 29(1): 27-32
  • McTernan, P.G., et al. Insulin and rosiglitazone regulation of lipolysis and lipogenesis in human adipose tissue in vitro. Diabetes2002, 51(5): 1493-1498
  • Lutoslawska, G., et al. Relationship between fasting insulin resistance index (FIRI) and plasma glycerol and free fatty acid levels in physically active males and females. Biol. Sport 2006, 23: 341-351
  • Kos, K., et al. Secretion of neuropeptide Y in human adipose tissue and its role in maintenance of adipose tissue mass. Am. J. Physiol. Endocrinol. Metab. 2007, 293: E1335-E1340
  • Rhodes, P., et al. Adult-onset obesity reveals prenatal programming of glucose-insulin sensitivity in male sheep nutrient restricted during late gestation. PloS ONE 2009, 4(10): e7393

Related Products

  • Glycerol Controls

Standard included in the kit

Clinical Chemistry Panel

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Glutamate

Reagent | Glutamate

Key Benefits

Wide measuring range

The Randox Glutamate assay has a wide measuring range of 0.11 – 10 mmol/l

Exceptional precision

The Glutamate assay showed a precision of less than 3% CV

Excellent stability

Reconstituted stability of 28 days on board the analyser when stored at 2-8°C

Other Features

  • Colorimetric method
  • Lypohilised reagents
  • Stable to expiry when stored at 2-8⁰C
  • Measuring range 0.11-10 mmol/l
  • For Biotechnology Industrial Use. Not for use in diagnostic procedures.

Ordering Information

Cat NoSize    
GM13724 x 20mlEnquireKit Insert RequestMSDSBuy Online

Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers.  Contact us to enquire about your specific analyser.

Clinical Chemistry Panel

For more information or to view more reagents within the clinical chemistry panel, please click here


GLDH

Glutamate Dehydrogenase (GLDH)

Key benefits of the Randox Glutamate Dehydrogenase (GLDH) reagent

Wide measuring range

The Randox GLDH (DGKC) assay has a wide measuring range of 2.90 – 77.3 U/l which will comfortably detect levels outside of the healthy range

 

Exceptional correlation

The GLDH (DGKC) assay showed a correlation of r=0.99 against another commercially available method

Stable to expiry date

Stable to expiry date when stored at +15oC to +25oC.

Ordering information

Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers.  Contact us to enquire about your specific analyser.

What is GLDH assay used for?

GLDH measurements can be taken to evaluate the liver function. Elevated blood serum levels indicate liver damage. GLDH also plays an important role in the differential diagnosis of liver disease, especially in combination with aminotransferases.

Liver diseases such as toxic liver damage or hypoxic liver disease are characterised by high serum levels. In clinical trials, GLDH can also be used as a measurement for the safety of a drug.

The Randox GLDH is an optimised standard method according to the recommendations of the Deutsche Gesellschaft für Klinische Chemie. This procedure measures the non specific creep reaction.

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Gamma GT

Reagent | Gamma GT

Benefits of the Gamma GT Assay

Wide measuring range

The Randox Gamma GT assay has a wide measuring range of 3.9-1285 U/l which will comfortably detect levels outside of the healthy range

Exceptional correlation

The assay showed a correlation of r=0.99 against another commercially available method

Excellent stability

Stable to expiry when stored at 2-8⁰C

Randox Gamma GT (Colorimetric)

  • Colorimetric method
  • Liquid and lypohilised reagents available
  • Stable to expiry when stored at 2-8⁰C
  • Measuring range 3.9-1285 U/l

Ordering Information

Cat NoSize    
GT3817R1 6 x 51ml
R2 6 x 14ml (L)
EnquireKit Insert RequestMSDSBuy Online
GT38746 x 21ml (L)EnquireKit Insert RequestMSDSBuy Online
GT8320R1 4 x 20ml
R2 4 x 7ml
(Mod. IFCC) (L)
EnquireKit Insert RequestMSDSBuy Online
GT8146R1 7 x 20ml (L)
R2 7 x 8ml
EnquireKit Insert RequestMSDSBuy Online
(L) Indicates liquid option

Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers.  Contact us to enquire about your specific analyser.

What is Gamma GT assay used for?

Gamma-glutamyltransferase (GT) in serum originates primarily from the hepatobiliary system. Therefore GT is elevated in all forms of liver disease and has been shown to be more sensitive than alkaline phosphatase in detecting obstructive jaundice, cholangitis and cholecystitis. High levels of GT are also seen in patients with primary or secondary liver cancer. Increased levels are also observed in cases of alcohol abuse and in alcoholic liver cirrhosis. In patients receiving anticonvulsant drugs such as phenytoin and phenobarbital, increased levels of the enzyme in serum may reflect induction of new enzyme activity and the toxic effects of alcohol and other drugs on the microsomal structures in liver cells. GT is the most sensitive enzymatic indicator of hepatobiliary disease, and can be used in combination with other biochemical markers to discriminate between different types of hepatobiliary disease.

  • Haçariz, O. et al. The effect of Quil A adjuvant on the course of experimental Fasciola hepatica infection in sheep. Vaccine 2009, 27(1): 45-50
  • Haçariz, O. et al. IL-10 and TGF-β1 are associated with variations in fluke burdens following experimental fasciolosis in sheep. Parasite Immunol. 2009, 31(10): 613-622
  • Gbadegesin, M.A., et al. In vitro antioxidant/radical scavenging activities and hepatoprotective roles of ethanolic extract of Cassia occidentalis leaves in sodium arsenite-treated male Wistar rats. Br. J. Med. Med. Res. 2013, 3(4): 2141-2156

Clinical Chemistry Panel

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CO2 Total Reagent

Reagent | CO2 Total

 

Key Benefits of the Randox CO2 Total Reagent

Exceptional correlation with standard methods

The Randox methodology was compared against other commercially available methods and the Randox CO2 Total assay showed a correlation coefficient of r=0.94

Wide measuring range

The healthy range for CO2 Total is 18 – 28 mmol/l. The Randox CO2 Total assay can comfortably detect levels outside of this healthy range measuring between 0.004 – 50 mmol/l

Suitable for use on a range of automated analysers

The Randox CO2 Total reagent is suitable for use on a number of third party automated analysers. To enquire about an Instrument Specific Application (ISA), please click the Contact Us button below.

Other features

  • Enzymatic method
  • Available as liquid and lyophilised reagents
  • Stable until expiry date when stored at +2 to +8°C
  • Open vial stability of 14 days at +10°C
  • Measuring range 0.004 – 50 mmol/l

Ordering information

Cat NoSize    
CD40064 x 21.7ml (C)EnquireKit Insert RequestMSDSBuy Online
(C) Indicates calibrator included in kit

Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers.  Contact us to enquire about your specific analyser.

What is CO2 Total used for?

Carbon dioxide (CO2) is a metabolic waste product of cellular respiration. It is transported in the bloodstream to the lungs and expelled from the body. CO2 exists in the body in two forms: 90% exists as bicarbonate (HCO3) and the remaining exists as carbonic acid (H2CO3) or dissolved CO2. The kidneys and lungs are responsible for the regulation on CO2, H2CO3 and HCO3 in the blood.

What is the CO2 Total assay used for?

The Randox CO2 Total assay is used for the quantitative in vitro determination of CO2 in serum and plasma.  It aids in diagnosing diseases associated with high and low levels of CO2 in the bloodstream.

Slightly elevated levels of CO2 do not have any serious consequences on the body, but overexposure to CO2 due to decreased alveolar ventilation or the inhalation of CO2 enriched air can cause serious implications in the body including: deterioration of respiratory functions due to respiratory acidosis and asphyxiation, cardiovascular effects due to low blood pressure and cardiac arrhythmia and nerve damage due to hypercapnia and acidemia.

For more information on the contrasting effects of hypoxia and hypercapnia, please click here.

High CO2 levels usually indicates that the lungs are not functioning properly and are unable to expel the required amount of CO2. During an acute illness, the levels of CO2 can increase suddenly, however, over time, some people are able to establish a new ‘baseline’ for CO2. An example of this is a person with stable chronic obstructive pulmonary disease (COPD) This means that the body is able to function with higher than normal levels of CO2.

There are some medical conditions and drugs that can cause low CO2 levels including: kidney disease as the kidneys are unable to carry out their functions, diabetic ketoacidosis due to the production of ketones resulting in reduced CO2 levels, hyperchloremic acidosis due to diarrhea, Addison’s disease, and metabolic acidosis due to chemical toxicity.

Clinical Chemistry Panel

For more information or to view more reagents within the clinical chemistry panel, please click here

Veterinary Panel

For more information or to view more reagents within the veterinary panel, please click here


Zinc Assay

Reagent | Zinc

Benefits of the Randox Zinc Assay

Excellent correlation

A correlation coefficient of r=0.9946 was displayed when the Randox method was compared against other commercially available methods.

Excellent precision

The Randox zinc assay displayed a within run precision of <3.87%.

Liquid ready-to-use

The Randox zinc assay is available in a liquid ready-to-use format for convenience and ease-of-use.

Standard supplied with the kit

The standard is supplied with the zinc kit, simplifying the ordering process.

Controls available

Controls available offering a complete testing package.

Applications available

Applications available detailing instrument-specific settings for the convenient use of the Randox zinc assay on a variety of clinical chemistry analysers.

Ordering Information

Cat NoSize    
ZN2341R1 1 x 50ml (S)
R2 1 x 250ml
with Deproteinisation
EnquireKit Insert RequestMSDSBuy Online
ZN26076 x 50ml
Deproteinising Solution
EnquireKit Insert RequestMSDSBuy Online
(S) Indicates standard included in kit

Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers.  Contact us to enquire about your specific analyser.

Physiological Significance

Zinc (ZN) is an essential trace element (micronutrient) and plays a vital role in several biological processes 1. ZN is released from food as free ions during digestion. Specific transport proteins facilitate the passage of ZN across cell membranes into circulation. 70% of circulatory ZN is bound to albumin 2. As ZN does not attain redox properties, it is capable of transportation around the biological systems without inducing oxidative damage, which can occur with other essential trace elements like copper 3.

ZN has a key role in growth, reproduction, sexual maturity and the immune system. ZN is vitally important in the functionality of >300 enzymes utilised in the stabilisation of DNA and gene expression 1. ZN can constitute strong, yet readily available flexible and exchangeable, complexes with organic molecules, enabling it to modify the three-dimensional structure of specific proteins, nucleic acids, and cellular membranes, thereby influencing the catalytic properties of many enzyme systems and intracellular signalling. ZN is associated with >50 metalloenzymes with a diverse range of functions and so ZN plays a central role in metabolism, differentiation and cellular growth 3.

Deficiency

Zinc deficiency has been identified as a malnutrition issue worldwide. ZN deficiency is more prevalent in areas of low animal consumption and high cereal consumption. It’s not that the diet is low in ZN but more so the bio-availability of ZN which plays a major role in its absorption. Phytic acid has been identified as the main inhibitor of ZN. Adolescents, children, infants, lactating women and pregnant women have increased requirements for ZN and so are at higher risk of zinc depletion. During growth periods, ZN deficiency causes growth failure. The organs most affected by ZN deficiency include: central nervous system, epidermal, gastrointestinal, immune, reproductive and skeletal systems 2.

Toxicity

As there are multiple sources of ZN in the environment, exposure to and toxicity from ZN are not uncommon. Case reports have documented zinc toxicity caused by: overuse of dietary supplements, inhalation from occupational sources, denture cream and ingestion of pennies, to which some of these cases had fatal outcomes 4.

It is believed that ZN toxicity from acute exposure differs significantly from chronic toxicity. In acute exposures, ingestions of ZN sulfate and concentrated ZN chloride will primarily result in gastrointestinal symptoms, such as haematemesis. Renal injury, liver necrosis, coagulopathy and even death have been reported following acute exposures 4.

Chronic exposure caused by excessive consumption of ZN, resulting in copper deficiency can lead to myelodysplastic syndrome, granulocytopenia and sideroblastic anaemia 4.

Clinical Chemistry Calibrator

Clinical Chemistry Control

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