Reagent | Bilirubin (Vanadate Oxidation)
A Superior Method for Paediatric & Lipemic Samples
Benefits of the Randox Vanadate Oxidation Bilirubin Assay
The Randox vanadate oxidation bilirubin assay does not suffer from interferences from non-conjugated bilirubin unlike the diazo-based methods.
Direct & Total bilirubin assays available
Randox offer both direct & total bilirubin assays offering choice and flexibility.
No pre-step required
The Randox vanadate oxidation bilirubin assay eliminates the pre-step, increasing testing efficiency.
The Randox bilirubin assay is available in a liquid ready-to-use format for convenience and ease-of-use.
Calibrator and dedicated control available
Calibrator and a dedicated bilirubin elevated control available offering a complete testing package.
Applications available detailing instrument-specific settings for the convenient use of the Randox vanadate oxidation bilirubin assay on a variety of clinical chemistry analysers.
Direct vanadate oxidation bilirubin
Total vanadate oxidation bilirubin
Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers. Contact us to enquire about your specific analyser.
Bilirubin is a metabolite of haem, derived from haem-containing proteins, including myoglobin, haemoglobin and various P450 enzymes, that serves to coordinate iron in various proteins. Half of this bilirubin is excreted into bile and the rest contributes to blood bilirubin turnover 1. The production of bilirubin mainly occurs in the spleen and liver but can also occur elsewhere in the body by macrophages and renal tubular cells 2. The internal hydrogen bonding causes bilirubin to be water-insoluble and so required enzyme-mediated glucuronidation in the liver for biliary excretion. Under normal conditions, bilirubin in mostly unconjugated and bound tightly to albumin 3.
Bilirubin is one of the most common tests to diagnose and monitor hepatic dysfunction. Bilirubin is impaired in cholestatic and parenchymal liver diseases. The most common condition associated with elevated bilirubin levels is jaundice (a clinical sign of hyperbilirubinaemia), characterised by the yellow colouring of skin, mucous membranes and sclera 3.
Today, bilirubin is recognised as being more than just an end-product and considered to be a fundamental substance. Bilirubin is recognised as acting as an antioxidant and anti-inflammatory agent in serum. Evidence exists that states that bilirubin can neutralise free radicals, prevent peroxidation of lipids and protects the cardiovascular system, hepatobiliary system, immune system, neuronal system and pulmonary system 4.
The main advantage of the vanadate oxidation method over the diazo method is that the V.O method is a superior method for haemolytic and lipemic samples. The advantages are particularly evident in neonatal and infant populations where haemolysis is common. Moreover, the V.O method offers a wider analytical measurement range 5.
It has been established that bilirubin levels are significantly elevated in COVID-19 patients 6. Patients with abnormal liver function tests were at a significantly higher risk of progressing to a severe disease, such as pneumonia. The presence of abnormal liver function tests became more pronounced during hospitalisation within two weeks, with total bilirubin levels elevated 3 times the upper normal limit 7.
Clinical Chemistry Calibrator
Bilirubin Elevated Control
 Leach T. Bilirubin Metabolism and Jaundice. https://almostadoctor.co.uk/encyclopedia/bilirubin-metabolism-and-jaundice (accessed 10 March 2020).
 Dhungana N, Morris C, Krasowski M.D. Operational impact of using a vanadate oxidase method for direct bilirubin measurements at an academic medical center clinical laboratory. Practical Laboratory Medicine 2017; 8(2017): 77-85.