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Next generation sequencing (NGS) offers speed, scalability, and ultra-high throughput. The technology can be used to determine the order of nucleotides in entire genomes or targeted regions of DNA/RNA. NGS has applications in environmental, agricultural, and forensic science as well as genomic and clinical research.

NGS Workflow

Extraction of genomic DNA and sample QC

Library Preparation

Next Generation Sequencing

Data analysis and report

NGS Capabilities

Whole Exome Sequencing

Widely used method that sequences every protein coding region to identify variants that may increase the risk of certain conditions. Can be used across a broad range of applications including cancer studies, genetic conditions, and population genetics. Provides a cost effective alternative to whole-genome sequencing.

Whole Genome Sequencing

Involves the sequencing of every single base in your DNA to identify variants that may increase the risk of certain conditions. Has many applications including genetic disorders, cancer, and monitoring disease outbreaks. Detects all variant types providing a high resolution view of the genome.

16srRNA

This method involves analysis of prokaryotic 16s ribosomal RNA found in bacteria and archaea.  This approach is extremely useful for microbiome research studies and has applications in environmental, clinical and food.

Microbial Genome Sequencing

Shotgun sequencing reads all the genomic DNA in the sample, rather than just one specific region.  This method provides vast amounts of genetic information and can identify archaea, bacterial, fungi and viruses. 

qPCR

qPCR allows for the analysis of variants at specific locations.  It is a good choice when interrogating a small region of DNA on a limited number of samples.  RTPCR can be used for both qualitative and quantitative analysis. 

Advantages and Applications of qPCR

chevron_right Reactions are run, and data is evaluated in a unified, closed-tube qPCR system.

chevron_right qPCR assays can be used to precisely monitor changes in gene expression.

chevron_right Highly sensitive and reliable variant detection.

chevron_right Quick and Simple Workflow

chevron_right Primary equipment already available in most labs.

Next Generation Sequencing (NGS) Advantages

High sample throughput

High sensitivity

Comprehensive genomic coverage

Can sequence thousands of genes simultaneously

Low sample input required

Fast turnaround time for high sample volumes

Get in touch to discover more

To find out more about next generation sequencing, enquire now.