Reagent | Aspartate Aminotransferase (AST)

A Marker of Hepatocellular Injury

Benefits of the Randox Aspartate Aminotransferase (AST) assay

Precision

Excellent precision

The Randox AST assay displayed a within run precision of < 4.96%.

Stability

Excellent stability

The Randox AST assay is stable to expiry when stored at +2oC to +8oC.

Liquid ready-to-use

Liquid ready-to-use

The Randox AST assay is available in a liquid ready-to-use format for convenience and ease-of-use.

Calibrator & Controls

Calibrator and controls available

Calibrator and controls available offering a complete testing package.

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Applications available

Applications available detailing instrument-specific settings for the convenient use of the Randox AST assay on a variety of clinical chemistry analysers.

Ordering Information

Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers.  Contact us to enquire about your specific analyser.

Cat NoSize
AS3804R1 6 x 51ml (L)
R2 6 x 14ml
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AS101R1 1 x 100ml (L)
R2 1 x 100ml
(Colorimetric, manual only)
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AS8005R1 6 x 56ml (L)
R2 6 x 20ml
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AS8306R1 4 x 20ml (L)
R2 4 x 7ml
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(L) Indicates liquid option

Clinical Significance

  • PHYSIOLOGICAL SIGNIFICANCE
  • Hepatic Function
  • Muscular Dystrophy
  • COVID-19

Enzymes are organic molecules responsible for the acceleration of biochemical reactions, however, emerge unchanged following the reaction. Aminotransferases are a family of enzymes that catalyse the conversion of amino acids to 2-oxo-acids by the transfer of amino acids 1. AST is present in mitochondrial and cytosolic enzymes (80% and 20% of activity respectively), found in brain, cardiac muscle, kidneys, leucocytes, liver, lungs, red blood cells and skeletal muscle 2.

AST is a marker of hepatocellular injury, predominantly alcohol-related liver injury (chronic hepatitis C) and cirrhosis (chronic hepatitis B). In alcoholic liver disease, P-5-P becomes deficient, which is greater on ALT activity compared to AST activity. Consequently, ALT activity is reduced, whereas AST activity is increased 2. The hallmark finding for alcohol liver disease is the AST to ALT ratio of at least 2:1 3. The marked laboratory findings for ischaemic hepatitis is an elevated bilirubin level, however, AST levels are > 10 times the upper reference range limit 2. Acute viral hepatitis, drug or toxin induced liver disease and ischaemic liver injury are characterised by extremely elevated aminotransferase levels 3.

Muscular dystrophy, including Duchenne muscular dystrophy is characterised by hypertransaminasemia. Elevations in both ALT and AST are most striking in the early stages of muscular disease, prior to the onset or only when subtle symptoms are present. Consequently, during these initial stages, ALT/AST testing can enable the early identification of disease and so the early intervention of treatment plans 4.

The diagnosis of liver disease in COVID-19 patients can be challenging for the clinician. There is often uncertainty as to whether there was a pre-existing undiagnosed liver disease. Also, many medications utilised to treat moderate and severe disease have their own profiles of liver toxicity. Elevations of aminotransferase is the most common abnormality in patients presenting with COVID-19. It was identified that AST is more frequently elevated in comparison to ALT 5. AST showed statistically significant elevations in severe COVID-19 in comparison to mild cases 6.

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