Key Benefits of the Bile Acids reagent
The Randox Bile Acids method is linear up to a concentration of 188 µmol/l. In the event of a rerun, the linearity is extended to 225 µmol/l
Exceptional correlation with standard methods
The Randox methodology was compared against other commercially available methods and the Randox Bile Acids assay showed a correlation coefficient of 0.99
Liquid ready-to-use reagent
The Randox Bile Acids reagent comes in a two shot ready-to-use liquid format which is more convenient as the reagent does not need to be reconstituted which aids in reducing the risk of errors occurring
Other features of the Randox Bile Acids reagent
- Liquid and lyophilised reagents available
- Stable to expiry when stored at +2 to +8°C
- Measuring range 1.47 – 188 µmol/l
- Protocols are available for a range of analysers
|(L) Indicates liquid option|
|BI2672||R1 10 x 10ml|
R2 1 x 30ml
|BI3863||R1 2 x 18ml (L)|
R2 2 x 8ml (5th generation)
|BI7982||R1 6 x 50ml (L)|
R2 6 x 18ml (5th generation)
Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers. Contact us to enquire about your specific analyser.
What is the Bile Acids assay used for?
What are bile acids?
Bile acids are water-soluble and, when ionized, amphipathic end products of cholesterol metabolism formed in the liver. In their molecular form, they are weak acids, however, after the biosynthesis process, the bile acid molecules are conjugated with glycine and taurine, converting them into strong amino acids before being excreted from the hepatocyte.
The fundamental role of bile acids is to aid in the digestion and absorption of fats and fat-soluble vitamins in the small intestine. For more information on intestinal digestion and absorption in humans, please click here [external link]. In doing so, bile acids have five physiological functions within the body:
- Elimination of cholesterol
- Transportation of lipids
- Stimulation of bile flow
- Stimulation of biliary phospholipid secretion
- Negative feedback regulation of bile acid and cholesterol biosynthesis
What is the bile acids assay used for?
Fasting serum bile acids can be used in the diagnosis and prognosis of liver disease. Levels rise in many liver diseases, for example hepatitis and liver sclerosis. Abnormal levels in fasting patients or immediately after a meal can be used to detect liver disease and damage, impaired liver function, intestinal dysfunction and perhaps a gall bladder blockage. Their measurement may detect some forms of liver disease earlier than standard liver tests because bile acids levels correspond to liver function, rather than liver damage. In veterinary medicine, bile acid measurement is considered to be a superior indicator of liver disease.
The fifth Generation Randox Total Bile Acids assay uses liquid stable two shot reagents in an enzymatic colorimetric assay for the quantitative in vitro determination of Bile Acids in serum or EDTA Heparinized plasma. The assay incorporates a more complex enzyme cycling mechanism than previous generations to amplify the signal, improving sensitivity and precision and reducing interference in haemolytic and lipaemic samples.