Key Benefits of the Randox G6PDH Reagent
The Randox Glucose-6-phosphate dehydorgenase (G6PDH/G6PD) test does not suffer from interferences due to the sample prewash step
Excellent correlation with standard methods
The Randox methodology has been compared against other commercially available methods and the Randox G6PDH assay showed a correlation coefficient of r=0.9903
The Randox G6PDH assay has a reconstituted stability of 4 weeks when stored at +2 – +8⁰C
Other features of the Randox G6PDH reagent
- UV method
- Lyophilised reagents
- Reconstituted stability of 4 weeks when stored at +2 – +8⁰C
- Measuring range 161 – 1232 U/l
What is the G6PDH assay used for?
What is G6PDH?
Glucose-6-phosphate dehydrogenase (G6PDH/G6PD) is a cytosolic enzyme located on the X-chromosome and can be found in every bodily cell. Glucose-6-phosphate dehydrogenase is involved in the normal processing of carbohydrates. G6PDH plays a critical role in red blood cells (RBC’s), protecting them from damage and premature destruction. The two main products of G6PDH are ribose-5-phosphate which is important for DNA, the chemical cousin of RNA. The chemical reaction produces NADPH which protects bodily cells from reactive oxygen species.
What is the G6PDH assay used for?
Glucose-6-phosphate dehydrogenase (G6PDH/G6PD) deficiency is the most common enzyme deficiency in the pentose phosphate pathway, affecting more than 400 million people globally. G6PDH deficiency is an X-linked recessive disorder mainly affecting red blood cells (RBC’s).
A defect in the G6PDH enzyme results in premature haemolysis (break down of RBC’s). If the bone marrow cannot compensate for the reduction of RBC’s, haemolytic anaemia can occur. Many individuals that are glucose-6-phosphate dehydrogenase deficient are asymptomatic most of the time, however when they are exposed to certain triggering factors, they can develop acute haemolytic anaemia (AHA), which can be life-threatening, especially in children. Symptoms associated with G6PDH deficiency can include paleness, jaundice, dark urine, fatigue, shortness of breath, a sudden rise in body temperature, lower back pain, splenomegaly (enlarged spleen) and a rapid heart rate. Other symptoms can include nausea, diarrhoea or abdominal discomfort. It has been noted that glucose-6-phosphate dehydrogenase deficiency is a significant cause of mild to severe jaundice in new-borns. For more information on haemolytic anaemia, please click here [external link]. Early and accurate diagnosis of glucose-6-phosphate dehydrogenase deficiency is essential in ensuring the successful management of haemolytic anaemia.
The Randox G6PDH assay is used for the quantitative in vitro determination of glucose-6-phosphate dehydrogenase in erythrocytes. The enzyme activity is determined by measurement of the rate of absorbance change at 340 nm due to the reduction of NADP+.
- Bildik, A., et al. The effect of hyperthyroidism on the levels of Na+K ATP+ase, glucose 6 phosphate dehydrogenase and glutathione. Israel J. Vet. Med. 2002, 57(2): 19-22
- Ainoon, O., et al. Glucose-6-phosphate dehydrogenase (G6PD) variants in Malaysian Malays. Hum Mutat. 2003, 21(1): 101
- Heidarpour, M., et al. Effect of long-term onion (Allium cepa) feeding on antioxidant enzymes in goat erythrocyte. Iranian Journal of Veterinary Research. 2013, 14(1): 21-28
- Isaac, I.Z., et al. Glucose-6-phosphate dehydrogenase deficiency among children attending the Emergency Paediatric Unit of Usmanu Danfodiyo University Teaching Hospital, Sokoto, Nigeria. International Journal of General Medicine. 2013, 6: 557-562