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COVID-19 | qPCR Assay

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COVID-19 | qPCR Assay

Posted on 10th November 2020 at 11:04 am.

Written by Martin Conway

COVID-19 | qPCR Assay

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Detecting SARS-CoV-2 (COVID-19)

Accurate PCR Detection of SARS-CoV-2

Validated for use on either the Applied Biosystems™ QuantStudio™ 5 (0.2 mL 96 well block) or Applied Biosystems™ 7500

High throughput: 96 samples including controls from extraction to analysis from automated nucleic acid isolation

CE marked assay detecting all known SARS-CoV-2 variants of concern

Multiplex PCR assay accurately detecting both SARS-CoV-2 (ORF1ab) and Sarbecovirus (E-gene)

Rapid turnaround time & PCR run time of 70 minutes

High sensitivity and specificity with 98% agreement. Low LOD: 250 copies/mL

Randox Laboratories have recently developed a COVID-19 qPCR assay, which can be used for the detection of nucleic acid from SARS-CovV-2 (ORF1ab) and Severe Acute Respiratory Syndrome ((SARS)/SARS-like) viruses (E-gene). PCR based assays are recommended to include two tests. One to detect a universal SARS-like gene and a secondary confirmatory test for gene sequences exclusive to SARS-CoV-2.

In a multiplex format, the Randox qPCR assay allows for differentiation of SARS-CoV-2 and SARS-like corona infections from other seasonal respiratory illnesses caused by viruses to prevent rapid spread of infection through the isolation of positive cases.

Our assay is compatible with sample matrices including nasal / throat swabs. This test is intended for use in the clinical setting as an aid in the screening and isolation of individuals that require quarantine and immediate intervention.

Internal control provided to monitor assay/sample integrity through the extraction and PCR process.

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Want to know more?

Contact us or visit our COVID-19 Monitoring & Management page

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COVID-19 Management & Monitoring

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