Bilirubin

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Bilirubin

Bilirubin

A Marker of Hepatic Dysfunction

Benefits of the Randox Bilirubin Assay

Correlation

Excellent correlation

The Randox bilirubin assay has a correlation coefficient > 0.99% CV when compared to commercially available methods.

Direct-Total

Direct & Total assays available

Randox offer both direct & total bilirubin assays offering choice and flexibility.

Liquid ready-to-use

Liquid ready-to-use

The Randox bilirubin assay is available in a liquid ready-to-use format for convenience and ease-of-use.

Calibrator & Controls

Calibrator and dedicated control available

Calibrator and dedicated control available offering a complete testing package.

Applications available

Applications available detailing instrument-specific settings for the convenient use of the Randox bilirubin assay on a variety of clinical chemistry analysers.

  • Ordering Information
  • Physiological Significance
  • Clinical Significance
  • COVID-19

Direct

Cat NoSizeMethod
BR1234562 x 250 (L)Modified JendrassikEnquireKit Insert RequestMSDSBuy Online
BR3807R1 2 x 30ml (L)
R2 8 x 4ml
JendrassikEnquireKit Insert RequestMSDSBuy Online
(L) Indicates liquid option

Total

Cat NoSizeMethod
BR23612 x 250ml (L)Modified JendrassikEnquireKit Insert RequestMSDSBuy Online
BR3859R1 2 x 50ml (L)
R2 8 x 4ml
JendrassikEnquireKit Insert RequestMSDSBuy Online
(L) Indicates liquid option

Direct & Total

Cat NoSizeMethod
BR411R1. 1 x 50ml (L)
R2. 1 x 10ml
R3. 1 x 100ml
R4. 1 x 100ml
JendrassikEnquireKit Insert RequestMSDSBuy Online

Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers.  Contact us to enquire about your specific analyser.

Bilirubin is a metabolite of haem, derived from haem-containing proteins, including myoglobin, haemoglobin and various P450 enzymes, that serves to coordinate iron in various proteins. Half of it is excreted into bile and the rest contributes to blood bilirubin turnover 1. It is mainly produced in the spleen and liver but can also occur elsewhere in the body by macrophages and renal tubular cells 2. The internal hydrogen bonding causes it to be water-insoluble and so required enzyme-mediated glucuronidation in the liver for biliary excretion. Under normal conditions, it is mostly unconjugated and bound tightly to albumin 3.

Bilirubin is one of the most common diagnostic tests utilised in the diagnosis and monitoring of hepatic dysfunction. It is impaired in cholestatic and parenchymal liver diseases. The most common condition associated with elevated levels is jaundice (a clinical sign of hyperbilirubinaemia), characterised by the yellow colouring of skin, mucous membranes and sclera 3.

Today, it is recognised as being more than just an end-product and considered to be a fundamental substance. Bilirubin is recognised as acting as an antioxidant and anti-inflammatory agent in serum. Evidence exists that states that it can neutralise free radicals, prevent peroxidation of lipids and protects the cardiovascular system, hepatobiliary system, immune system, neuronal system and pulmonary system 4.

It has been established that bilirubin levels are significantly elevated in COVID-19 patients 5. Patients with abnormal liver function tests were at a significantly higher risk of progressing to a severe disease, such as pneumonia. The presence of abnormal liver function tests became more pronounced during hospitalisation within two weeks, with total bilirubin levels elevated 3 times the upper normal limit 6.

Related Products

Clinical Chemistry Calibrator

Bilirubin Elevated Control

Reagents Homepage


Bilirubin (Vanadate Oxidation)

Reagent | Bilirubin (Vanadate Oxidation)

A Superior Method for Paediatric & Lipemic Samples

Benefits of the Randox Vanadate Oxidation Bilirubin Assay

Superior Performance

Superior methodology

The Randox vanadate oxidation bilirubin assay is a superior method for haemolytic and lipaemic samples.

 

Direct-Total

Direct & Total bilirubin assays available

Randox offer both direct & total bilirubin assays offering choice and flexibility.

No Pre-step

No pre-step required

The Randox vanadate oxidation bilirubin assay eliminates the pre-step, increasing testing efficiency.

Liquid ready-to-use

Liquid ready-to-use

The Randox bilirubin assay is available in a liquid ready-to-use format for convenience and ease-of-use.

Calibrator & Controls

Calibrator and dedicated control available

Calibrator and a dedicated bilirubin elevated control available offering a complete testing package.

Applications available

Applications available detailing instrument-specific settings for the convenient use of the Randox vanadate oxidation bilirubin assay on a variety of clinical chemistry analysers.

  • Ordering Information
  • Physiological Significance
  • Clinical Significance
  • V.O vs Diazo
  • COVID-19

Direct Vanadate Oxidation Bilirubin

Cat NoSize
BR9765R1 4 x 14ml
R2 4 x 6ml
EnquireKit Insert RequestMSDSBuy Online
BR8133R1 4 x 52.2
R2 4 x 20ml
EnquireKit Insert RequestMSDSBuy Online
BR4060R1 4 x 20ml
R2 4 x 20ml
EnquireKit Insert RequestMSDSBuy Online
BR8308R1 4 x 20ml
R2 4 x 8ml
EnquireKit Insert RequestMSDSBuy Online
BR8376R1 4 x 20ml
R2 4 x 8ml
EnquireKit Insert RequestMSDSBuy Online

Total Vanadate Oxidation Bilirubin

Cat NoSize
BR9766R1 4 x 68ml
R2 4 x 25ml
EnquireKit Insert RequestMSDSBuy Online
BR4061R1 4 x 20ml
R2 4 x 8ml
EnquireKit Insert RequestMSDSBuy Online
BR8132R1 4 x 52.2ml
R2 4 x 20ml
EnquireKit Insert RequestMSDSBuy Online
BR8377R1 4 x 20ml
R2 4 x 8ml
EnquireKit Insert RequestMSDSBuy Online

Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers.  Contact us to enquire about your specific analyser.

Bilirubin is a metabolite of haem, derived from haem-containing proteins, including myoglobin, haemoglobin and various P450 enzymes, that serves to coordinate iron in various proteins. Half of this bilirubin is excreted into bile and the rest contributes to blood bilirubin turnover 1. The production of bilirubin mainly occurs in the spleen and liver but can also occur elsewhere in the body by macrophages and renal tubular cells 2. The internal hydrogen bonding causes bilirubin to be water-insoluble and so required enzyme-mediated glucuronidation in the liver for biliary excretion. Under normal conditions, bilirubin in mostly unconjugated and bound tightly to albumin 3.

Bilirubin is one of the most common tests to diagnose and monitor hepatic dysfunction. Bilirubin is impaired in cholestatic and parenchymal liver diseases. The most common condition associated with elevated bilirubin levels is jaundice (a clinical sign of hyperbilirubinaemia), characterised by the yellow colouring of skin, mucous membranes and sclera 3.

Today, bilirubin is recognised as being more than just an end-product and considered to be a fundamental substance. Bilirubin is recognised as acting as an antioxidant and anti-inflammatory agent in serum. Evidence exists that states that bilirubin can neutralise free radicals, prevent peroxidation of lipids and protects the cardiovascular system, hepatobiliary system, immune system, neuronal system and pulmonary system 4.

The main advantage of the vanadate oxidation method over the diazo method is that the V.O method is a superior method for haemolytic and lipemic samples. The advantages are particularly evident in neonatal and infant populations where haemolysis is common. Moreover, the V.O method offers a wider analytical measurement range 5.

It has been established that bilirubin levels are significantly elevated in COVID-19 patients 6. Patients with abnormal liver function tests were at a significantly higher risk of progressing to a severe disease, such as pneumonia. The presence of abnormal liver function tests became more pronounced during hospitalisation within two weeks, with total bilirubin levels elevated 3 times the upper normal limit 7.

Related Products

Clinical Chemistry Calibrator

Bilirubin Elevated Control

Reagents Homepage


Alanine Aminotransferase (ALT) Reagent

Alanine Aminotransferase (ALT) Reagent

Key Benefits of the Alanine Aminotransferase (ALT) reagent

Flexibility

Liquid and lyophilised reagents available to offer greater customer choice

Suitable for use on a range of analysers

Alanine Aminotransferase (ALT) Reagent is suitable to use on a number of third party analysers Including Abbott, Olympus, Cobas and Hitachi.

Stability

ALT is stable for up to 14 days when stored at +2 to +8°C

More features of the Alanine Aminotransferase (ALT) reagent

  • IFCC method
  • Available as liquid and lyophilised reagents
  • Stable to for up to 14 days when stored at +2 to +8°C

Ordering information

Cat NoSize
AL1205R1a. 1 x 105ml
R1b. 10 x 10ml
EnquireKit Insert RequestMSDSBuy Online
AL7930R1 7 x 100ml (L)
R2 3 x 60ml
EnquireKit Insert RequestMSDSBuy Online
AL3801R1 6 x 51ml (L)
R2 6 x 14ml
EnquireKit Insert RequestMSDSBuy Online
AL8006R1 6 x 56ml (L)
R2 6 x 20ml (Mod. IFCC)
EnquireKit Insert RequestMSDSBuy Online
AL8304R1 4 x 20ml (L)
R2 4 x 7ml (Mod ?IFCC)
EnquireKit Insert RequestMSDSBuy Online
(L) Indicates liquid option

Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers.  Contact us to enquire about your specific analyser.

Randox ALT-Colorimetric ordering information

Cat NoSize
AL146R1. 1 x 100ml
R2. 1 x 100ml
R3. 1 x 100ml
EnquireKit Insert RequestMSDSBuy Online
(S) Indicates standard included in kit

Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers.  Contact us to enquire about your specific analyser.

What is ALT assay used for?

Alanine Aminotransferase is one of the enzymes within the aminotransferases group and are among the most sensitive liver enzymes. The highest concentrations of the ALT enzyme can be in the liver, with decreasing concentrations found in the kidneys, heart, skeletal muscle, pancreas, spleen and lung tissue respectively.

ALT measurements are used in the diagnosis of hepatic damage and diseases including viral hepatitis and cirrhosis.

The normal concentration levels of ALT in the blood are low, however, when damaged, the liver releases more ALT into the blood causing the concentration levels to rise.  When diagnosing for hepatic damage, the root cause of the damage can be established, such as disease, drug, or injury

The ALT test not only allows for the diagnosis of liver disease but also allows for the diagnosis of the root cause of the disease

It is often tested in combination with the aspartate aminotransferase (AST) test as part of the hepatic panel with alanine aminotransferase levels being higher in most types of liver disease.  For more information on the validity and clinical utility of AST and ALT when assessing disease severity, please click here

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