Glutathione Peroxidase (Ransel)
Glutathione Peroxidase (Ransel)
Reagent | Glutathione Peroxidase (Ransel)
A Marker of Oxidative Stress
Key Benefits
Superior method
The Randox Ransel assay utilises the enzymatic method enabling the sensitive and accurate detection of glutathione peroxidase.
Excellent precision
The Randox Ransel assay displayed a within run precision of <4.86% CV.
Excellent correlation
A correlation coefficient of r=0.9829 was displayed when the Randox Ransel assay was compared to commercially available methods.
Excellent measuring range
The Randox Ransel has a measuring range of 75 – 925U/l for the comfortable detection of clinically important results.
Dedicated calibrator and control available
Dedicated Ransel calibrator and control available offering a complete testing package.
Applications available
Applications available detailing instrument-specific settings for the convenient use of the Randox Ransel assay on a variety of clinical chemistry analysers.
Ordering Information
| Cat No | Size | ||||
|---|---|---|---|---|---|
| RS504 | 8 x 6.5ml | Enquire | Kit Insert Request | MSDS | Buy Online |
| RS505 | 8 x 10ml | Enquire | Kit Insert Request | MSDS | Buy Online |
Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers. Contact us to enquire about your specific analyser.
Diagnostic Uses
Glutathione peroxidase (GPx) is a reactive oxygen species (ROS), generated by bodily cells via mitochondrial and enzymatic sources. It is vital that GPx levels are reviewed as it can cause oxidative damage to membrane lipids, proteins and DNA. GPx is an intracellular antioxidant enzyme that enzymatically converts hydrogen peroxide to water (detoxification). Hydrogen peroxidase is vital for the maintenance of normal thiol redox-balance, mitochondrial function, and growth factor-mediated signal transduction. By converting hydrogen peroxidase to water, GPx aids in modulating these processes 1. GPx also contributes to cell signalling and oxidative protein folding 2.
Four of the eight glutathione peroxidases (GPx) are selenoproteins (containing the amino acid form of selenium, Sec) in humans, however, GPx6 is cysteine-containing in rodents, but a selenoprotein in humans, whereas the remaining GPx’s are cysteine containing. Glutathione peroxidase 4 (GPx4) is emerging as one of the most important seleoproteins in mammals and is one of the key regulators of ferroptosis, a form of regulated necrotic cell death 2.
GPx displays an inverse correlation with free radicals and consequently oxidative stress (OS). When GPx activity is reduced, antioxidant protection is impaired, resulting in oxidative damage to the membrane fatty acids and functional proteins, resulting in neurotoxic damage. Decreased GPx activity and OS is implicated in the incidence and progression of several health problems, including: cardiovascular disease (CVD), diabetes, atherosclerosis, neurodegenerative disorders and other chronic conditions 3.
Ransel Calibrator
Ransel Control
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References
Superior Performance & Unique Tests
Niche & Unique Reagents
Randox offer a range of high performance, unique and niche reagents that are designed to enhance your laboratory testing capabilities.
Our impressive portfolio of high performance & unique tests together with our standard assays sets us apart in the in vitro diagnostics market. Our superior performance reagents and methodologies deliver highly accurate and specific results, that can facilitate earlier diagnosis of disease states with confidence and precision.
Benefits of High Performance Reagents
Reduce Costs
We can help create cost-savings for your laboratory through excellent stability, eliminating the requirement for costly test re-runs. Our quality reagents also come in a range of different kit sizes to reduce waste and for your convenience.
Confidence in Patient Results
Our traceability of material and extremely tight manufacturing tolerances ensure uniformity across our reagent batches. All of our assays are validated against gold-standard methods.
Applications Available
Applications are available detailing instrument-specific settings for the convenient use of the Randox superior performance & unique assays on a wide variety of clinical chemistry analysers.
Superior Performance Offering
Randox offer an extensive range of 115 assays across routine and niche tests, and cover over 100 disease makers. Our high performance assays deliver superior methodologies, more accurate and specific results compared to traditional methods.
Reduce Labour
Reduce valuable time spent running tests. Randox reagents come in liquid ready-to-use formats and various kit sizes for convenient easy-fit. Barcode scanning capabilities for seamless programming.
Unique Offering
Our range of unique assays means that Randox are one of the only manufacturers to offer these tests in an automated biochemistry format.
The in vitro diagnostics market is continuously adapting to the changes in laboratory testing. Consequently, Randox have continued to reinvest in R&D to produce superior performance & unique tests offering laboratories choice, quality and innovation.
The Randox Lp(a) assay is calibrated in nmol/l and traceable to the WHO/IFCC reference material (IFCC SRM 2B) and provides an acceptable bias compared with the Northwest Lipid Metabolism Diabetes Research Laboratory (NLMDRKL) gold standard. A five-point calibrator with accuracy-based assigned target values (in nmol/l) is available, accurately reflecting the heterogeneity of the apo(a) isoforms.
The Randox bile acids test utilises an advanced enzyme cycling method which displays outstanding sensitivity and precision when compared to traditional enzymatic based tests. The Randox 5th Generation Bile Acids test is particularly useful in paediatrics where traditional bile acids tests are affected by haemolytic and lipaemic samples.
A superior assay from Randox, the vanadate oxidation method offers several advantages over the diazo method, including less interference by haemolysis and lipaemia, which is particularly evident for infant and neonatal populations.
Adiponectin has been identified as having pleiotropic functions widely associated with anti-atherogenic, anti-diabetic, cardioprotective and anti-inflammatory effects. Adiponectin levels inversely correlate with insulin levels, BMI, triglyceride levels, insulin resistance (IR), glucose, and most importantly, visceral fat accumulation.
Soluble transferrin receptor (sTfR) is a marker of iron status. In iron deficiency anaemia, sTfR levels are significantly increased, however remain normal in the anaemia of inflammation. Consequently, sTfR measurement is useful in the differential diagnosis of microcytic anaemia.
The Randox Fructosamine assay utilises the enzymatic method which offers improved specificity and reliability compared to conventional NBT-based methods. The Randox enzymatic method does not suffer from non-specific interferences unlike other commercially available fructosamine assays.
Current challenges facing our healthcare systems
Chronic Kidney Disease (CKD)
Worldwide 1/5 of men and 1/4 of women between 65 and 74 years of age have Chronic kidney disease (CKD). CKD is an umbrella term encompassing a wide range of renal conditions from commonly prevalent sub-clinical, asymptomatic to rare end-stage renal disease requiring dialysis or a transplant to sustain life. Kidney disease is ranked in stages from stage 1 (very mild damage) through to stage 5 (kidney failure) 7. Symptoms are commonly expressed in the later stages of renal impairment, however, at this point dangerous levels of fluid, electrolytes and waste products can build up inside the body. The aim of CKD treatment is to slow the progression of the disease, thus early intervention is vital 8.
Type 2 Diabetes Mellitus
425 million people are living with type 2 diabetes mellitus (T2DM) and 352 million are at risk of developing T2DM. T2DM is a serious condition whereby blood glucose levels are elevated (hyperglycaemia). T2DM is characterised by insulin resistance or insulin deficiency. T2DM is the most common form of diabetes, accounting for 90% of cases. The key to T2DM is control. Implementing lifestyle changes, oral medication and in more severe cases, insulin, a diabetic can take control of their disease, keeping glucose levels stable. When glucose levels are not monitored and controlled, associated complications may arise including: diabetic nephropathy, CVD and renal impairment 5, 6.
Cardiovascular Disease (CVD)
CVD accounts for 45% of all deaths in europe and 37% of all deaths in the EU. Atherogenesis is a circulatory disease whereby atheromas are formed (plaque build-up) within the artery. Plaque is a combination of cholesterol, fat, calcium, lipids and other substances within the blood stream. As time progresses, the plaque hardens, narrowing the arteries. This is known as atherosclerosis. Consequently, blood flow through the narrowed artery is reduced, limiting the supply of blood to vital organs and bodily tissues. As atherogenesis can affect any artery within the body, different diseases may develop based on the artery that is affected. Such diseases include: coronary heart/artery disease, carotid artery disease, peripheral artery disease and chronic kidney disease 2, 3, 4.
References
[2] European Heart Network (EHN). European Cardiovascular Disease Statistics 2017. http://www.ehnheart.org/cvd-statistics/cvd-statistics-2017.html (accessed 16 April 2019).
[3] National Heart, Lung, and Blood Institute (NIH). https://www.nhlbi.nih.gov/health-topics/atherosclerosis (accessed 16 April 2019).
[4] Falk E. Why do plaques rupture? Circulation 1992; 86(6): 11130-11142.
[5] Diabetes UK. What is Type 2 diabetes? https://www.diabetes.org.uk/diabetes-the-basics/what-is-type-2-diabetes (accessed 16 April 2019).
[6] American Diabetes Association. Type 2 Diabetes. https://www.diabetes.org/diabetes/type-2 (accessed 16 April 2019).
[8] Mayo Clinic. Chronic kidney disease. https://www.mayoclinic.org/diseases-conditions/chronic-kidney-disease/symptoms-causes/syc-20354521 (accessed 16 April 2019).
Glutathione Peroxidase (Ransel) Quality Control
A dedicated Glutathione Peroxidase control for use in the routine monitoring of the Randox Ransel assay on clinical chemistry analysers.
Features & Benefits
- Lyophilised for enhanced stability
- Bovine based whole blood
- Stable to expiry date at 2°C – 8°C
- Reconstituted stability of 3 days at 2°C – 8°C
| Description | Size | Analytes | Cat No | |
|---|---|---|---|---|
| Ransel (glutathione peroxidase) control | 10 x 1 ml | 1 | SC692 | |
Analytes
- Glutathione Peroxidase