Bile Acids Reagent

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Bile Acids Reagent

Bile Acids Reagents

Features & Benefits of the Randox Bile Acids reagents

Excellent linearity

The Randox Bile Acids method is linear up to a concentration of 150 µmol/l

Exceptional correlation with standard methods

The Randox methodology was compared against other commercially available methods and the Randox Bile Acids assay showed a correlation coefficient of 0.99

Flexibility

Liquid and lyophilised reagents available for greater customer choice

 

Analyser protocols

Protocols are available for a range of analysers

Excellent stability

Stable to expiry when stored at +2 to +8°C

Ordering information

Cat NoSize
BI3863 (5th)R1 2 x 18ml (L)
R2 2 x 8ml
EnquireKit Insert RequestMSDSBuy Online
BI7982 (5th)R1 6 x 50ml
R2 6 x 18ml
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BI8150 (5th)R1 2 x 17.7ml (L)
R2 2 x 8.9ml
EnquireKit Insert RequestMSDSBuy Online
(L) Indicates liquid reagent

Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers.  Contact us to enquire about your specific analyser.

What are Bile Acids used for?

  • Clinical Significance
  • Biological Significance
  • Inadequacies of traditional bile acid assays

Liver Function

Measuring total bile acid (TBA) levels may prove useful for the detection of liver diseases such as viral hepatitis, mild liver injury through drug use and for further evaluation of patients with chronic hepatitis who were previously treated successfully. TBA levels may rise up to 100 times the normal concentration in patients with liver disease due to impairment of hepatic synthesis and extraction of bile acids. Measurement of TBA in serum can be used in the diagnosis and prognosis of liver diseases and may detect some forms of liver disease earlier than standard liver markers due to the correlation of TBA with liver function, rather than liver damage.

Bile Acid Deficiency

TBA deficiency is caused by a genetic error in one of the 17 enzymes that produce bile acids. Deficiency can lead to liver failure and even death in infants, therefore early detection is vital. People with TBA deficiency may exhibit symptoms, including:

• Vitamin deficiencies, specifically of fat-soluble vitamins such as A, D, E, and K
• Jaundice
• Stunted or abnormal growth
• Diarrhoea
• Loss of liver function
• Liver failure

Intrahepatic Cholestasis of Pregnancy

Intrahepatic cholestasis of pregnancy (ICP) or obstetric cholestasis is a pregnancy-specific liver disorder. It can be indicated by pruritus, jaundice, elevated TBA levels and/or serum transaminases and usually affects women during the second and third trimester of pregnancy. ICP is a condition that restricts the flow of bile through the gallbladder resulting in a build-up of TBA in the liver. Due to the build-up, Bile Acids leak into the bloodstream where they are detected at concerning levels. It is an extremely serious complication of pregnancy that can lead to the increased risk of premature birth or even stillbirth as such it is vital that women with the condition are monitored carefully.

According to several reports TBA levels in ICP can reach as high as 100 times the upper limit of a normal pregnancy. It has been reported that a doubling in maternal serum TBA levels, results in a 200% increased risk of stillbirth. Additionally, bile acids can affect the foetal cardiovascular system as it has been found that there are often cardiac rhythm disturbances in the foetus due to the elevated TBA in circulation.

There are several risk factors associated with ICP such as family history, use of oral contraceptives, assisted reproduction techniques and multiple gestation. Genetic influence accounts for approximately 15% of ICP cases. Dietary selenium is a contributing environmental factor as serum selenium levels often decrease throughout pregnancy. Further to this, incidences of ICP rise in the winter months and are most likely due to the fact selenium levels are naturally less during these months. In healthy pregnancies, there is very little increase in TBA levels although a slight increase is likely to be seen in the third trimester.

Measurement of TBA in serum is thought to be the most suitable method of diagnosing and monitoring ICP.

Bile acids are water-soluble and amphipathic end products of cholesterol metabolism formed in the liver. Bile is stored in the gall bladder and released into the intestine when food is consumed. The fundamental role of bile acids is to aid in the digestion and absorption of fats and fat-soluble vitamins in the small intestine. In doing so, bile acids have five physiological functions within the body as shown below:

Determining the cause and extent of liver damage is important in guiding treatment decisions and preventing disease progression. Standard liver function tests include; ALT, AST, ALP, GGT and Bilirubin. The measurement of TBA is most beneficial in conjunction with these standard liver tests and offers unrivalled sensitivity allowing identification of early stage liver dysfunction.

There are several commercial methods available for the detection and measurement of TBA in serum. Traditional TBA tests based on the enzymatic method use nitrotetrazolium blue (NBT) to form a formazan dye. The reaction is measured at 546nm and the intensity of the colour is proportional to the concentration of bile acids.

Newer methods such as the enzyme cycling method or fifth generation methods offer many advantages including greater sensitivity, liquid reagents, small sample volumes and reduced instrument contamination from formazan dye. Additionally, the fifth generation assay does not suffer from interference from lipaemic or haemolytic samples. Both lipaemia and haemolysis are common in new-borns and pregnant women.

Enzyme cycling methods offer superior analytical performance, two reactions are combined. In the first reaction, bile acids are oxidised by 3-α hydroxysteroid dehydrogenase with the subsequent reduction of Thio-NAD to Thio-NADH. In the second reaction, the oxidised bile acids are reduced by the same enzyme with the subsequent oxidation of NADH to NAD. The rate of formation of Thio-NADH is determined by measuring the specific absorbance change at 405nm. Enzyme cycling means multiple Thio-NAD molecules are generated from each bile acid molecule giving rise to a much larger absorbance change and signal amplification, increasing the sensitivity of the assay.

The assay principle is demonstrated in the diagram below:

The Randox fifth generation assay utilises the advanced enzyme cycling method which displays outstanding sensitivity and precision compared to traditional enzymatic based tests. The assay shows excellent linearity of up to 188 µmol/l with the normal upper range of TBA in a fasting serum sample being at 10 µmol/l. The liquid ready-to-use reagent is available along with complementary controls and calibrators for a complete testing package.


Apolipoprotein A-II

Reagent | Apolipoprotein A-II

Key benefits of the Randox Apolipoprotein A-II reagent

Exceptional measuring range

The Randox albumin assay has a measuring range 6.75-61.1 mg/dl for the comfortable detection of results.

Excellent stability

Stable to expiry when stored at 2-8°C

Liquid ready-to-use reagents

The Randox Apolipoprotein A-II reagent comes in a liquid format which is more convenient, and can also help reduce the risk of errors occurring

Applications available

Applications available detailing instrument-specific settings for a variety of clinical chemistry analysers.

Ordering information

Cat NoSize
LP3867R1 2 x 11ml
R2 2 x 5ml
EnquireKit Insert RequestMSDSBuy Online

Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers.  Contact us to enquire about your specific analyser.

What is Apolipoprotein A-II assay used for?

Apolipoproteins are proteins on the surface of the lipoprotein complex that bind to specific enzymes or transport proteins on the cell membranes. This directs the lipoprotein to the proper site of metabolism. APO A-II is mainly created in the liver and modulates lipoprotein lipase and hepatic triglyceride lipase. It also acts as a co-factor for Lecithin Cholesterol Acyltransferase which is involved in lipoprotein processing.

Apolipoprotein A-II is a major constituent of High Density Lipoprotein (HDL) particles and plays an important role in the reverse cholesterol transport and lipid metabolism. The APO A-II test can be used as an aid in assessing the risk of CVD.


Apolipoprotein A-I

Reagent | Apolipoprotein A-I

Key benefits of the Randox Apolipoprotein A-I reagent

Liquid ready-to-use reagents

 Liquid format which is more convenient, and can also help reduce the risk of errors occurring

Excellent stability

The Randox Apolipoprotein A-I reagent is stable to expiry when stored at 2-8°C

Applications available

Applications available detailing instrument-specific settings for a variety of clinical chemistry analysers.

Ordering information

Cat NoSize
LP2116R1 4 x 40ml (C) (L)
R2 4 x 17ml
EnquireKit Insert RequestMSDSBuy Online
LP3838R1 4 x 30ml (L)
R2 4 x 12ml
EnquireKit Insert RequestMSDSBuy Online
(C) Indicates calibrator included in kit
(L) Indicates liquid reagent

Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers.  Contact us to enquire about your specific analyser.

What is Apolipoprotein A-I assay used for?

The Apolipoproteins are the main form of protein found in High Density Lipoproteins (HDL). The main role of APO A-I is in the activation of Lecithin Cholesterol Acyl Transferase (LCAT) and removal of free cholesterol from extra hepatic tissues. APO A-I may therefore be described as non atherogenic, showing an inverse relationship to cardiovascular risk.

APO A-I may be measured in patients with a personal or family history of high concentrations of lipids and/or premature CHD. It may be requested to find out the cause of high lipid levels and/or a suspected disorder that is causing a deficiency in APO A-I. APO A-I can be used with APO B-100 to check your ratio of “good” to “bad” cholesterol

Publications



    sTfR Assay

    Reagent | Soluble Transferrin Receptor

    Key benefits of the Randox sTfR assay

    Excellent correlation
    correlation coefficient of r=0.977 was displayed when the Randox methodology was compared against other commercially available methods.

    Latex Enhanced Immunoturbidimetric method
    Facilitating testing on biochemistry analysers and eliminating the need for dedicated equipment

    Excellent measuring range
    The healthy range for sTfR is 0.65 – 1.88mg/L. The Randox sTfR assay can comfortably detect levels outside of the healthy range, measuring between 0.5 – 11.77 mg/L.

    Liquid ready-to-use assay
    The Randox sTfR assay is available in a liquid ready-to-use format for convenience and ease-of-use.

    Stable to expiry date
    The Randox sTfR assay is stable to expiry date when stored at +2 to +8°C

    Applications are available
    Applications are available detailing instrument-specific settings for the convenient use of the Randox soluble transferrin receptor assay on a wide range of clinical chemistry analysers.

    Ordering information

    Cat NoSize
    TF10159R1 1 x 9ml (L)
    R2 1 x 5.8ml
    EnquireKit Insert RequestMSDSBuy Online
    (L) Indicates liquid option
    • Clinical Significance
    • Biological Significance
    • Completing your anaemia toolbox

    The most common nutritional deficiency globally is iron deficiency (ID) with 15% to 20% of the global population affected by IDA 1. IDA has also been recognised as the most common form of anaemia in infants aged between 4 and 24 months of age, school-age children, female adolescents, pregnant women, and nurturing mothers. It has been recognised that young children including infants require high levels of iron during growth periods making them more susceptible to ID 2.

    Soluble transferrin receptor levels have also been found to be a strong biomarker of erythropoietic and haemolysis drive compared to iron-restricted erythropoiesis in paediatric sickle cell disease 3.

    Another study found that elevated levels of sTfR is linked with central obesity in men with hyperferritinemia 4.

    sTfR has also “been included in multivariable blood testing models for the detection of performance enhancing erythropoietin misuse in sport” and has been recommended as a marker of ID in athletes 5.

    A truncated extracellular form of the membrane transferrin receptor, soluble transferrin receptor (sTfR) is a marker of iron status and erythropoiesis. sTfR levels have been found to increase in iron-deficient erythropoiesis and iron deficiency anaemia (IDA). Some have reported that sTfR is useful in the differential diagnosis of IDA and anaemia of chronic disease or inflammation (ACD)1.

    At present, ferritin remains the traditional iron deficiency marker with serum ferritin reflecting intracellular iron storage. Ferritin is an acute phase reactant, and so ferritin levels can be influenced by inflammatory conditions. In the presence of inflammation, ferritin levels may be represented as an elevated value leading to a false representation of iron stores in the body, resulting in a delayed diagnosis. For these reasons, sTfR should be tested when the reliability of a ferritin test is compromised.

    There isn’t a single diagnostic test to diagnose anaemia. Currently the diagnosis comprises of two steps:

    > Firstly confirming that the patient has anaemia which utilises the haemoglobin assay and red blood cell count.

    > Secondly is the determination of the root cause of the anaemia which can be identified through testing sTfR and transferrin levels.

    Randox offer a number of diagnostic reagents in addition to sTfR which can be used on a wide range of biochemistry analysers for the diagnosis of anaemia. The Randox assays have shown clinical utility in testing for anaemia. The Randox anaemia toolbox comprises of: iron, ferritin, transferrin, unsaturated iron binding capacity (UIBC), total iron binding capacity (TIBC) and the recent addition of sTfR.

    There are many different types of anaemia and many triggers and conditions which contribute to the development of anaemia. For this reason, a single test cannot diagnose an individual with anaemia while also knowing what the root cause of it is. Additionally, patient symptoms can give an indication but a full range of diagnostic tests provide independent information to aid the definitive medical diagnosis and enable clinicians to provide effective treatment for patients.

     

    Related products

    sTfR Control

    sTfR Calibrator

    Resource Hub

    • References

      [1] Freixenet, N. et al., 2009. Serum soluble transferrin receptor concentrations are increased in central obesity. Results from a screening programme for hereditary hemochromatosis in men with hyperferritinemia. 400(1-2).

      [2] Lulla, R., Thompson, A. & Liem, R., 2010. Elevated soluble transferrin receptor levels reflect increased erythropoietic drive rather than iron deficiency in pediatric sickle cell disease.

      [3] Monajemzadeh, S. M. & Zarkesh, M. R., 2009. Iron deficieny anemia in infants aged 12-15 months in Ahwaz, Iran. 52(2).

      [4] Schumacher, Y., Schmid, A., König, D. & Berg, A., 2002. Effects of exercise on soluble transferrin receptor and other variables of the iron status.

      [5] Yoon, S. H. et al., 2015. The usefulness of soluble transferrin receptor in the diagnosis and treatment of iron deficiency anemia in children.


    CK-NAC Reagent

    Reagent | CK-NAC

    Key Benefits of the Randox CK-NAC reagent

    Exceptional correlation with standard methods

    The Randox methodology was compared against other commercially available methods and the Randox CK-NAC assay showed a correlation coefficient of r=0.99

    Excellent stability

    Stable until expiry date when stored at +2 to +8°C

    Other features of the Randox CK-NAC reagent

    • UV DGKC method
    • Available as liquid and lyophilised reagents
    • Stable until expiry date when stored at +2 to +8°C

    Ordering information

    Cat NoSize
    CK110R1a 1 x 70ml
    R1b 20 x 2.5ml
    EnquireKit Insert RequestMSDSBuy Online
    CK3812R1a 2 x 70ml
    R1b 4 x 20ml
    R2 4 x 6ml
    EnquireKit Insert RequestMSDSBuy Online
    CK3892R1 4 x 16.5ml (L)
    R2 4 x 6.2ml (IFCC)
    EnquireKit Insert RequestMSDSBuy Online
    CK8313R1a 2 x 70ml (L)
    R1b 4 x 20ml
    R2 4 x 7ml
    EnquireKit Insert RequestMSDSBuy Online
    (L) Indicates liquid option

    Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers.  Contact us to enquire about your specific analyser.

    What is the CK-NAC assay used for?

    What is CK-NAC?

    The small amount of Creatine Kinase (CK) present in the blood primarily comes from skeletal muscle. CK is also found in striated muscle, brain and heart tissues. The CK concentration levels in the blood increase as a result of damage to bodily muscle or interferences with the muscle energy production such as myositis, which is inflammation of the muscles.

    What is the CK-NAC assay used for?

    The Randox CK-NAC reagent is used for the measurement of CK in serum or plasma to assess the extent of muscle damage and to monitor treatment.

    The determination of CK-NAC activity in plasma or serum provides a sensitive marker for the detection of skeletal muscle disease; and is also useful to assess the extent of severe muscle trauma, crush injuries, burns or electrocution, and the likelihood of developing rhabdomyolysis.   For more information on the spectrum of rhabdomyolysis, please click here [external link].

    The determination of CK using creatine phosphate and adenosine‑5’‑diphosphate (ADP) as substrates rather than creatine and adenosine‑5’‑triphosphate (ATP) has several advantages in test performance as it allows for a faster reaction rate resulting in greater sensitivity. Small sample volumes are used and sample blanks are not required.

    The UV method used is an optimised standard method according to the recommendations of the Deutsche Gesellschaft für Klinische Chemie.

    Publications


      Clinical Chemistry Panel

      For more information or to view more reagents within the clinical chemistry panel, please click here.

      Veterinary Panel

      For more information or to view more reagents within the veterinary panel, please click here

      Publications


        CK-MB Reagent

        Reagent | CK-MB

        Key Benefits of the Randox CK-MB reagent

        Liquid and lyophilised reagents available

        The Randox CK-MB reagent is available in a liquid ready-to-use or in a lyophilized format offering you convenience and choice when selecting the best choice for your laboratory

        Excellent stability

        Stable until expiry date when stored at +2 to +8°C

        Other features of the Randox CK-MB reagent

        • Immunoinhibition UV method
        • Available as liquid and lyophilised reagents Stable until expiry date when stored at +2 to +8°C
        • Stable until expiry date when stored at +2 to +8°C
        Cat NoSize
        CK129619 x 2.5mlEnquireKit Insert RequestMSDSBuy Online
        CK3813R1 2 x 70ml
        R1b 4 x 20ml
        R2 4 x 6ml
        EnquireKit Insert RequestMSDSBuy Online
        CK4043R1 4 x 20ml (L)
        R2 4 x 6ml
        EnquireKit Insert RequestMSDSBuy Online
        CK8148R1 4 x 20ml (L)
        R2 4 x 8ml
        EnquireKit Insert RequestMSDSBuy Online
        (L) Indicates liquid option

        Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers.  Contact us to enquire about your specific analyser.

        What is the CK-MB assay used for?

        What is CK-MB?

        Creatine Kinase (CK) is internationally accepted as a sensitive and specific indicator of acute myocardial infarction (AMI). There are 3 major forms of the iso-enzymes of CK, CK-MM, CK-MB and CK-BB. CK-BB is produced by the brain in very small insignificant amounts. CK-MM is produced by the skeletal and heart tissue. CK-MB is produced by the heart muscle and increases when the heart muscle is damaged. For more information on the structure and function of CK isoenzymes in tissue, please click here.

        What is the CK-MB assay used for?

        CK-MB is a more sensitive marker to myocardial infarction than total CK activity as CK-MB has a lower basal level and a narrower normal range.  In the vast majority of cases the CK-MB activity rises within 4 to 6 hours of an acute infarction.  Between 10 to 24 hours, maximum values are observed. The CK-MB activity generally returns to normal between the third and fourth day post- infarction.

        The Randox CK-MB reagent can measure small, but significant changes in CK-MB activity during the early hours following the onset of chest pain.

        Publications


          Clinical Chemistry Panel

          For more information or to view more reagents within the clinical chemistry panel, please click here

          Cardiology Panel

          For more information or to view more reagents within the cardiology panel, please click here


          Featured Reagent – D-3-Hydroxybutyrate (Ranbut)

          Featured Reagent | D-3-Hydroxybutyrate

          Back to Reagents Resource Hub >

           

          Benefits of D-3-Hydroxybutyrate

          Superior Methodology – The commercially available nitroprusside method is a semi-quantitative dipstick test which only detects acetone and acetoacetate. As the most abundant ketone produced during ketosis, D-3-hydroxybutyrate is more sensitive and specific.

          Excellent precision – The Randox Ranbut assay displayed an excellent precision of <3.5%.

          Exceptional correlation – A correlation coefficient of r=0.9954 was displayed when the Randox method was compared against other commercially available methods.

          Wide Measuring Range – The Randox Ranbut assay has a measuring range of 0.100 – 5.75mmol/l for the comfortable detection of clinically important results.

          Calibrator and controls available – Randox offer a complete testing package.

          Applications available – Detail of instrument-specific settings for the convenient use of the Randox Ranbut assay on a variety of clinical chemistry analysers.

          Traditional Methods

          Ketogenesis is a biochemical process whereby the body produces ketone bodies (acetone, acetoacetate, beta-hydroxybutyrate). As ketone bodies are water soluble, they do not require lipoproteins for transport 1.

          In healthy humans, small amounts of ketones are continuously made for the body to use an energy source. Ketone bodies increase in times of fasting and sleeping 1.

          Physiological Significance

          Urinalysis is an essential part of the diagnostic review for kidney disease and other renal impairments. Whilst the dipstick test allows for rapid and simultaneous chemical analyses of urine, including ketones, the chemical reactions on dipsticks are complicated and can be affected by oxidising, reducing, and discolouring substances in urine. Therefore, false positives and false negative results are common in dipstick testing 4.

          Clinical Significance

          When the carbohydrate stores are significantly decreased, or the fatty acid concentration is increased, there is an upregulation of the ketogenic pathway and consequently, an increased production of ketone bodies. This is commonly observed in alcoholism, type I diabetes and starvation. Most organs, including the brain, can utilise ketones whereas the heart utilises fatty acids as its source of energy, but can also use ketones. The liver however, cannot utilise ketones, despite producing them as the liver lacks the necessary enzyme ketoacyl-CoA transferase 1.

          Ketosis is the presence of ketones. Whilst ketosis is not dangerous, if left untreated, especially in diabetes, ketoacidosis (high levels of ketones) develops 2.

          In type 1 diabetes mellitus (T1DM), the body is unable to produce insulin resulting in bodily cells not receiving energy from glucose, causing the body to release hormones to breakdown fat for energy, producing ketones. If left untreated, diabetic ketoacidosis develops, a serious health condition. Diabetic ketoacidosis is commonly triggered by an illness, infection or missing insulin treatments 3.

          Did you know?

          Ketone bodies increase in times of fasting and sleeping 1 and is considered harmless, however, if left untreated, especially in diabetes, ketoacidosis occurs 2.

          References

          [1] Dhillon KK, Gupta S. Biochemistry, Ketogenesis. Treasure Island: StatPearls Publishing; 2020. https://www.ncbi.nlm.nih.gov/books/NBK493179/ (accessed 28 September 2020).

          [2] Hecht M. Ketosis vs. Ketoacidosis: What You Should Know. https://www.healthline.com/health/ketosis-vs-ketoacidosis (accessed 28 September 2020).

          [3] Mayo Clinic. Diabetic ketoacidosis. https://www.mayoclinic.org/diseases-conditions/diabetic-ketoacidosis/symptoms-causes/syc-20371551 (accessed 28 September 2020).

          [4] Han TH. Urinalysis: The Usefulness and Limitations of Urine Dipstick Testing. Journal of the Korean Society if Pediatric Nephrology 2013; 17(2): 42-48.

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          Cholinesterase (Butyryl) (BCHE / BuCHE) Reagent

          Reagent | Cholinesterase (Butyryl) (BCHE / BuCHE)

          Key Benefits of the Cholinesterase (Butyryl) (BCHE / BuCHE) reagent

          Exceptional correlation with standard methods

          The Randox methodology was compared against other commercially available methods and the Randox cholinesterase (butyryl) reagent showed a correlation coefficient of r=0.9959

          Excellent stability

          Stable for 6 weeks when stored at +2 to +8°C

          Other features of the Cholinesterase (Butyryl) (BCHE / BuCHE) reagent

          • Colorimetric method
          • Lyophilised reagents
          • Correlation coefficient of r=0.99 when compared with another commercially available method
          • Stable for 6 weeks when stored at +2 to +8°C
          • Measuring range 455-28722U/l

          Ordering information

          Cat NoSize
          CE190R1 5 x 30ml
          R2 5 x 1ml
          EnquireKit Insert RequestMSDSBuy Online

          Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers.  Contact us to enquire about your specific analyser.

          What is the Cholinesterase (Butyryl) (BCHE / BuCHE) assay used for?

          What is cholinesterase (butyryl)?

          Cholinesterase (butyryl) (BCHE / BuCHE), also known as pseudocholinesterase, is a protein coding gene and is a serine hydrolase synthesised in the liver and is responsible for catalysing the hydrolysis of numerous choline esters.  It can be found in the liver, intestinal mucosa, plasma, pancreas and the white matter of the central nervous system of mammals.

          The Randox Cholinesterase (butyryl) reagent is used for the quantitative in vitro determination of BCHE in serum and plasma. BCHE can be used to diagnose cholinesterase (butyryl) deficiency and curariform drugs toxicity.

          The Randox Cholinesterase (butyryl) reagent can also be used as a diagnostic and therapeutic marker for Alzheimer’s disease as BCHE activity progressively increases in patients with Alzheimer’s disease whereas acetylcholinesterase activity remains the same or declines. In Alzheimer’s disease, BCHE is found in association with beta-amyloid plaques and the cerebral cortex, whereas it is not commonly found in any other case. A study carried out by NCBI found that when the BCHE gene is not found in the brain, there are up to 70% less beta-amyloid plaques. For more information on the diagnosis of Alzheimer’s disease, please click here [external link].

          Clinical Chemistry Panel

          For more information or to view more reagents within the clinical chemistry panel, please click here

          Veterinary Panel

          For more information or to view more reagents within the veterinary panel, please click here


          sdLDL Cholesterol Assay

          Reagent | Small Dense LDL Cholesterol (sdLDL-C)

          Size Matters: The True Weight of Risk in Lipid Profiling

          Benefits of the Randox sdLDL-C Assay

          Superior Performance

          Superior method

          The clearance method produces results in as little as ten minutes, facilitating faster patient diagnosis and treatment plan implementation. The previous methods, ultracentrifugation and electrophoresis, were laborious and time consuming.

          Exceptional correlation

          correlation coefficient of r=0.91 was displayed when the Randox methodology was compared against the gold standard method, ultracentrifugation.

          Liquid ready-to-use assay

          The Randox sdLDL-C assay is available in a liquid ready-to-use format for convenience and ease-of-use.

          Calibrator & Controls

          Dedicated calibrator and control available

          Dedicated sdLDL-C calibrator and control available offering a complete testing package.

          Applications available

          Applications are available detailing instrument-specific settings for the convenient use of the Randox sdLDL-C assay on a wide range of clinical chemistry analysers.

          • Ordering Information
          • Physiological Significance
          • Risk Assessment
          • Management
          • Methods of Detection
          Cat NoSize
          562616R1 1 x 19.8ml (L)
          R2 1 x 8.6ml
          EnquireKit Insert RequestMSDSBuy Online
          CH8153R1 1 x 16.2ml (L)
          R2 1 x 8.2ml
          EnquireKit Insert RequestMSDSBuy Online
          562760 (U)R1 1 x 18ml
          R2 1 x 7ml
          EnquireKit Insert RequestMSDSBuy Online
          562791 *(U)R1 5 x 200ml EnquireKit Insert RequestMSDSBuy Online
          562807 *(U)R2 2 x 200mlEnquireKit Insert RequestMSDSBuy Online
          (L) Indicates a liquid reagent
          (U) Indicates for use in the USA only
          (*) Indicates that boths kits must be purchased together

          Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers.  Contact us to enquire about your specific analyser.

          When measuring LDL cholesterol (LDL-C), it is the cholesterol mass within the LDL particles that is being measured. The LDL particle population within LDL is heterogeneous – meaning that the size, density & composition of each particle will be different. sdLDL-C is a subfraction of low density lipoprotein (LDL) with smaller particle size and higher density than larger more buoyant LDL. They all transport triglycerides and cholesterol to the tissues, but their atherogenesis varies according to their size. sdLDL-C will more readily permeate the inner arterial wall. sdLDL-C is more susceptible to oxidation and has a lower affinity to the hepatic LDL receptor, and as such circulates in the blood longer1.

          As sdLDL-C is particularly atherogenic, a person with elevated sdLDL-C levels has a 3-fold increased risk of myocardial infarction (MI)2.
          sdLDL-C measurement therefore provides a more comprehensive understanding of cardiovascular disease (CVD) risk compared to traditional LDL-C tests.

          sdLDL-C Scale

          Reducing sdLDL-C levels will aid in reducing the risk of CVD and MI. High dose statin therapy has been proven to aid in reducing the levels of sdLDL-C as a risk factor for cardiovascular events and high risk patients. Elevated levels of sdLDL-C arise from multiple sources. A major factor is a sedentary lifestyle with a diet high in saturated fat. Insulin resistance and pre-diabetes have also been implicated, in addition to genetic predisposition3.

          The measurement of LDL-C or the review of levels within arteriosclerotic coronary heart disease (ASCHD) treatment are known within different guidelines (including ATP III, AHA/ ACC, ESC/ EAS and NICE). However doubt remains on the impact of targeting LDL-C only. The inclusion of sdLDL-C within the clinical testing panel will assist in removing this doubt.

          The Randox sdLDL-C test is a direct method for the quantitative determination of sdLDL-C using a range of chemistry analysers capable of accommodating two-reagent assays. The assay consists of two steps and is based on the use of well-characterised surfactants and enzymes that selectively react with certain groups of lipoproteins.

          sdLDL-C Calibrator

          sdLDL-C Control

          Lipid EQA Scheme


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          In order to make your laboratory testing easier, Randox offers a range of clinical chemistry reagents with dedicated barcodes that are optimised for use on a range of specific clinical analysers.

          • All reagents are presented in bottles that fit directly onto the analyser
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          Randox Easy Fit reagents

          We are dedicated to providing reagents that fit perfectly with your laboratory needs. In addition to providing an extensive test menu of the highest quality reagents, we also ensure flexibility, convenience and ease-of-use with our Easy Fit reagents bottles.

          • We offer a range of clinical chemistry reagents that are optimised for use on an extensive range of clinical chemistry analysers
          • All reagents are presented in bottles which fit easily onto your analyser avoiding the need to pour reagents into dedicated bottles
          • Randox reagents offer increased accuracy and reliability
          • A wide range of validated analyser applications ensure ease of programming and confidence in results
          • A simple 2 step process allows you to actively run Randox reagents on your clinical chemistry analyser

          We also offer general applications that cover a range of requirements from manual use to use on multiple semi-automated and automated analyers.

          Email Us

          Get in touch with Randox via email at reagents@randox.com

          Need Instructions?

          Kit Inserts are available to download for free on our online portal

          Buy Online

          Order your lipid kits today by visiting our online store 

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