Apolipoprotein A-II

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Apolipoprotein A-II

Reagent | Apolipoprotein A-II

Key benefits of the Randox Apolipoprotein A-II reagent

Exceptional measuring range

The Randox albumin assay has a measuring range 6.75-61.1 mg/dl for the comfortable detection of results.

Excellent stability

Stable to expiry when stored at 2-8°C

Liquid ready-to-use reagents

The Randox Apolipoprotein A-II reagent comes in a liquid format which is more convenient, and can also help reduce the risk of errors occurring

Applications available

Applications available detailing instrument-specific settings for a variety of clinical chemistry analysers.

Cat NoSize    
LP3867R1 2 x 11ml
R2 2 x 5ml
EnquireKit Insert RequestMSDSBuy Online

Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers.  Contact us to enquire about your specific analyser.

What is Apolipoprotein A-II assay used for?

Apolipoproteins are proteins on the surface of the lipoprotein complex that bind to specific enzymes or transport proteins on the cell membranes. This directs the lipoprotein to the proper site of metabolism. APO A-II is mainly created in the liver and modulates lipoprotein lipase and hepatic triglyceride lipase. It also acts as a co-factor for Lecithin Cholesterol Acyltransferase which is involved in lipoprotein processing.

Apolipoprotein A-II is a major constituent of High Density Lipoprotein (HDL) particles and plays an important role in the reverse cholesterol transport and lipid metabolism. The APO A-II test can be used as an aid in assessing the risk of CVD.


Apolipoprotein A-I

Reagent | Apolipoprotein A-I

Key benefits of the Randox Apolipoprotein A-I reagent

Exceptional measuring range

The Randox albumin assay has a measuring range 5.27 – 251mg/dl for the comfortable detection of results.

Excellent stability

The Randox Apolipoprotein A-I reagent is stable to expiry when stored at 2-8°C

Applications available

Applications available detailing instrument-specific settings for a variety of clinical chemistry analysers.

Liquid ready-to-use reagents

 Liquid format which is more convenient, and can also help reduce the risk of errors occurring

Ordering information

Cat NoSize    
LP2116R1 4 x 40ml (C)
R2 4 x 17ml
EnquireKit Insert RequestMSDSBuy Online
LP3838R1 4 x 30ml
R2 4 x 12ml
EnquireKit Insert RequestMSDSBuy Online
LP8007R1 4 x 30ml
R2 4 x 12ml
EnquireKit Insert RequestMSDSBuy Online
(C) Indicates calibrator included in kit

Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers.  Contact us to enquire about your specific analyser.

What is Apolipoprotein A-I assay used for?

The Apolipoproteins are the main form of protein found in High Density Lipoproteins (HDL). The main role of APO A-I is in the activation of Lecithin Cholesterol Acyl Transferase (LCAT) and removal of free cholesterol from extra hepatic tissues. APO A-I may therefore be described as non atherogenic, showing an inverse relationship to cardiovascular risk.

APO A-I may be measured in patients with a personal or family history of high concentrations of lipids and/or premature CHD. It may be requested to find out the cause of high lipid levels and/or a suspected disorder that is causing a deficiency in APO A-I. APO A-I can be used with APO B-100 to check your ratio of “good” to “bad” cholesterol


Apolipoprotein E (ApoE) Assay

Reagent | Apolipoprotein E (ApoE)

A Genetic Risk Factor for CVD & Alzheimer’s Disease

Benefits of the Randox ApoE Assay

Correlation

Exception correlation

A correlation coefficient of r=1.00 was displayed when the Randox apoE assay was compared to commercially available methods.

Precision

Excellent precision

The Randox apoE assay displayed a precision of <2.79% CV.

Measuring range

Extensive measuring range

The Randox apoE assay has a measuring range of 1.04 – 12.3mg/dl for the comfortable detection of clinically important results.

Liquid ready-to-use

Liquid ready-to-use

The apoE assay is available in a liquid ready-to-use format for convenience and ease-of-use.

Calibrator & Controls

Dedicated calibrator and controls available

Randox offer dedicated apolipoprotein calibrator and controls for a complete testing package.

Applications available

Applications available detailing instrument-specific settings for the convenient use of the Randox apoE assay on a variety of clinical chemistry analysers.

  • Ordering Information
  • PHYSIOLOGICAL SIGNIFICANCE
  • CARDIOVASCULAR DISEASE
  • NEUROLOGICAL DISEASES
Cat NoSize    
LP3864R1 2 x 11ml
R2 2 x 5ml
EnquireKit Insert RequestMSDSBuy Online

Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers.  Contact us to enquire about your specific analyser.

The apolipoproteinE (APOE) gene provides instructions for the production of the apolipoprotein E (apoE) protein. The apoE protein binds with lipid forming lipoproteins which are responsible for the transportation of cholesterol and other lipids through the bloodstream 1.

Apolipoprotein E (apoE) is a multifunctional glycoprotein with central roles in lipid metabolism, neurobiology, and neurodegenerative diseases. ApoE has three major isoforms (apoE2, apoE3, and apoE4) all of which have different effects on lipid and neuronal homeostasis (fig 1). The key function of apoE is to mediate the binding of lipoproteins or lipid complexes in the plasma or interstitial fluids to specific cell-surface receptors. These receptors internalise apoE-containing lipoprotein particles and so apoE participates in the distribution or redistribution of lipids among various tissues and bodily cells 2. The e3 allele is the most of the three and may be considered an ancestral allele. The e4 allele is more common in those of Northern European ancestry and lower in those of Asian ancestry 3.

Fig. 1. ApoE isoforms and their properties 2

Both apoE2 and apoE4 alleles are associated with cardiovascular disease (CVD).

As apoE2 binds defectively to LDL receptors, apoE2 homozygosity can precipitate type III hyperlipoproteinemia, however, only occurs when another condition, including: diabetes, oestrogen deficiency, hypothyroidism, or obesity, leads to the overproduction of VLDL or fewer LDL receptors, overwhelming the limited ability of apoE2 to mediate the clearance of triglyceride-rich and cholesterol-rich β-VLDL. Other dominant and recessive mutations in apoE that affect residues in or around the receptor binding region also causes type III hyperlipoproteinemia 3.

ApoE3 increases LDL levels in plasma and the risk of atherosclerosis. The lipoprotein-binding preference of apoE4 to large (30-80nm), triglyceride-rick VLDL, is associated with elevated levels of LDL. The enrichment of VLDL with apoE4 accelerates their clearance from the plasma by receptor-mediated endocytosis in the liver and consequently, LDL receptors are downregulated, and LDL levels rise 3.

ApoE4 is the major genetic risk factor, or causative gene, for Alzheimer’s disease (AD) and other neurological disorders, including poor clinical outcomes following traumatic brain injury, stroke, frontotemporal dementia, Down syndrome, certain patients with Parkinson’s disease, and Lewy body disease 3.

Apo E4 drastically affects AD with 65-80% of all AD patients carrying at least one apoE4 allele. ApoE4 increases the risk of developing AS 4-fold (one allele) and 14-fold (two allele). Carrying one e4 allele is not uncommon with approximately 25% of people worldwide having at least one E4 allele. Fig. 2 illustrates the apoE-mediated pathogenic pathways leading to AD, with amyloid β playing a key role 3.

Fig. 2. ApoE-mediated pathogenic pathways leading to Alzheimer’s Disease 4

Related Products

Apolipoprotein Calibrator

Apolipoprotein Control

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IgE Assay

Reagent | Immunoglobulin (IgE)

A Marker of Allergic Diseases

Our Benefits

Correlation

Exceptional correlation

A correlation coefficient of r=1.00 was displayed when the Randox methodology was compared against commercially available methods.

Precision

Excellent precision

The Randox IgE assay displayed a precision < 4.0% CV.

Measuring range

Excellent measuring range

The Randox IgE assay has a measuring range of 19.6 – 1007IU/ml for the comfortable detection of clinically important results.

Liquid ready-to-use

Liquid ready-to-use

The Randox IgE assay is available in a liquid ready-to-use format for convenience and ease-of-use.

Calibrator & Controls

Calibrator and controls available

Dedicated IgE calibrator and specific protein controls available for a complete testing package.

Applications available

Applications available detailing instrument-specific settings for the convenient use of the Randox IgE assay on a variety of clinical chemistry analysers.

  • Ordering Information
  • PHYSIOLOGICAL SIGNIFICANCE
  • Clinical Significance
Cat NoSize    
IE7308R1 1 x 8ml
R2 1 x 5ml
EnquireKit Insert RequestMSDSBuy Online

Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers.  Contact us to enquire about your specific analyser.

Immunoglobulin E (IgE) is one of five classes of immunoglobulins (IgA, IgD, IgE, IgG and IgM). IgE was the last immunoglobulin to be discovered. However, since it’s discovery, vast amounts of research have been aimed at characterising its physiological and clinical significance’s. Whilst IgEs chemical structure is unique compared to the rest of the immunoglobulin family (lacks a ‘hinge’ region in the centre of the molecule and gets replaced by the C-epsilon2 domain), it has an array of physiological functions. For immunoglobulin E to fulfil its function, the Fc portion of the antibody must bind to a given cellular receptor located on certain cell types, such as eosinophil or mast cells. Whilst many an array of cellular receptors have been identified, the main ones are Fc-epsilon-RI, Fc-epsilon-II and CD23. Fc-epsilon-RI is the high affinity receptor located on basophils, dendritic cells, eosinophils, mast cells and macrophages and is responsible for immediate hypersensitivity reactions, enhanced cytokine production, parasitic immunity, and antigen presentation 1.

It is believed that immunoglobulin E evolved as a defence mechanism against parasitic infestation. The major sites of parasitic invasion are the gut, respiratory tract and skin, the typical allergic response sites. IgE antibodies play a key role in the early recognition of foreign material or a general potentiation of the immune system response through improved antigen presentation. An allergy triggered by IgE could be beneficial to the host as the typical allergic reactions include: sneezing, coughing, inflammation, bronchoconstriction and vomiting, to expel allergenic proteins from the body. Different allergens stimulate the production of corresponding allergen-specific immunoglobulin E antibodies 2. The antigen-dependent activation of tissue mast cells that have specific immunoglobulin E bound to their surface is the central event in acute allergic reactions. IgE specific allergens include: allergic or atopic asthma, atopic dermatitis (eczema), food allergies such as peanut and shellfish, allergic rhinitis (hay fever), house dust mite, latex allergy, dog or cat allergies 2, 3.

IgE Calibrator

Specific Proteins Controls

Immunoassay EQA

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Apolipoprotein C-II (Apo C-II) Assay

Reagent | Apolipoprotein C-II (Apo C-II)

In Association with Hypertriglyceridemia

Benefits of the Randox Apo C-II Assay

Superior method

The immunoturbidimetric method limits interference from Bilirubin, Haemoglobin, Intralipid® and Triglycerides, producing more accurate results.

Exceptional correlation

A correlation coefficient of r=1.00 was displayed when the Randox apo C-II assay was compared to commercially available methods.

Excellent measuring range

The Randox apo C-II assay has a measuring range of 1.48 – 9.70mg/dl for the comfortable detection of clinically important results.

Liquid ready-to-use

The Randox apo C-II assay is available in a liquid ready-to-use format for convenience and ease-of-use.

Dedicated calibrator and controls available

Randox offer dedicated apolipoprotein calibrator and controls for a complete testing package.

Applications available

Applications available detailing instrument-specific settings for the convenient use of the Randox apo C-II assay on a variety of clinical chemistry analysers.

  • Ordering Information
  • PHYSIOLOGICAL SIGNIFICANCE
  • Clinical Significance
Cat NoSize    
LP3866R1 2 x 11ml
R2 2 x 5ml
EnquireKit Insert RequestMSDSBuy Online

Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers.  Contact us to enquire about your specific analyser.

Apo C – II is a 79-amino acid protein synthesised in the liver and is the co-factor for lipid transport in the bloodstream 1. Apo C – II is a surface constituent of lipoproteins and the C – terminal helix activates lipoprotein lipase (LPL) 2. The active peptide of apo C – II corresponds to residues 44 – 79 and has been identified to reverse the symptoms of genetic apo C – II deficiency. Moreover, LPL is also a key enzyme in the regulation of triglyceride levels 3.

Both an excess and deficiency of apo C – II is associated with hypertriglyceridemia and reduced LPL activity. Elevated levels of apo C-II is associated with excess triglyceride – rich particles and altercations in the distribution of HDL particles, increasing the risk of CVD 4. Whilst extremely rare, a deficiency in apo C-II results in excess fasting hypertriglyceridemia and chylomicronemia. Hypertriglyceridemia can cause eruptive xanthomas, pancreatitis, hepatosplenomegaly and lipemia retinalis. Biologically and clinically, apo C – II deficiency closely mimics LPL deficiency. Synonyms for apo C-II deficiency include: C – II an apolipoproteinemia and hyperlipoproteinemia type Ib 5.

Apolipoprotein Calibrator

Apolipoprotein Control

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Assessing the risk of developing Alzheimer’s disease

World Alzheimer’s Month

World Alzheimer’s Month is a global campaign to raise awareness and highlight the challenge that surrounds the disease, hosted by the Alzheimer’s disease International (ADI) every September. During this month World Alzheimer’s Day also takes place, 21 September each year.

47 million people are living with Alzheimer’s worldwide, costing 604 billion USD per year. This number is expected to rise to 76 million people with the disease by 2030.1 The FDA have not approved a medication for the treatment of Alzheimer’s disease since 2003. More than 400 clinical trials are currently looking at new treatments for Alzheimer’s disease (AD) and many of them are actively recruiting. Many still regard the amyloid hypothesis as a key explanation for Alzheimers disease development and progression.2

Alzheimer’s risk

Alzheimer’s disease is not necessarily inherited as a single-gene mutation as the inheritance pattern is incredibly complex. Unlike familial Alzheimer’s disease, a multi-gene form usually affects those aged 65 and older. The gene with the greatest known effect on the risk of developing late-onset Alzheimer’s disease is called apolipoprotein E (APOE). It is found on chromosome 19 and the APOE protein plays a role in handling fats in the body, including cholesterol. 3

ApoE plays a key role in lipid metabolism and the scientific and medical community recognise it as one of the most powerful genetic risk factors for dementia and other neurodegenerative diseases. It has become one of the most widely studied gene variants in Alzheimer’s disease and constitutes a major consideration for preventive medicine.

ApoE exists in three common isoforms (ApoE2, ApoE3 and ApoE4) which are coded by three co-dominant alleles (e2, e3, e4). As such, six common ApoE phenotypes exist within the general population: E2/E2, E3/E3, E4/E4 (homozygous) and E2/E3, E2/E4, E3/E4 (heterozygous). Medical professionals recognise the presence of the ApoE4 isoform as a major genetic risk factor for development of Alzheimer’s disease. Therefore, the availability of analytical methods for rapid and reliable ApoE4 classification is advantageous.

Evidence Investigator

The Apolipoprotein E4 (ApoE4) Array is a research use only product developed for the Evidence Investigator. The ApoE4 Array measures both total ApoE protein levels and ApoE4 protein levels directly from plasma samples and using a ratio can classify patients as negative or positive for ApoE4. In turn we can then assess their risk for the  development of Alzheimer’s disease.

2-plex Biochip Array

  • Pan ApoE
  • ApoE4

An individual’s ApoE status has been shown to affect pre-symptomatic risk, diagnosis, prognosis, and treatment response for a variety of diseases, in particular Alzheimer’s disease. The ApoE4 Array can rapidly and accurately detect an individual’s ApoE4 status directly from a plasma sample. In combination with medical and family history, medication and lifestyle, this can deliver valuable information for personalised medicine approaches.

The 2-plex diagnostic Alzheimer’s test has the utility to detect the likelihood of a person’s chance of developing the disease to assist in the research and development of a potential drug to combat or slow down the process of Alzheimer’s.

1 https://www.alz.org/global/overview.asp

2 https://www.brightfocus.org/alzheimers/article/clinical-trials-alzheimers-disease-whats-new 

3 https://www.alzheimers.org.uk/about-dementia/risk-factors-and-prevention/alzheimers-disease-and-genes

 

For further information about the Randox Alzheimer’s Array, please email info@randoxbiosciences.com

 

 

 


Lipid Control Calibrators

Therapeutic Drug Quality Control

Apolipoprotein Calibrator

Multi-analyte, apolipoprotein calibrator with values provided for Apolipoprotein A-1 and Apolipoprotein B.

Features & Benefits

  • Lyophilised for enhanced stability
  • 100% human serum
  • Stable to expiry date at 2°C – 8°C
  • Reconstituted stability of up to 7 days at 2°C – 8°C
DescriptionSizeAnalytesCat No 
Apolipoprotein Calibrator3 x 1ml2LP3023

Analytes

  • Apo A-1
  • Apo B

Apolipoprotein Calibrator 2

Multi-analyte calibrator covering four specialised apolipoprotein assays. A reconstituted stability of up to 28 days helps to keep waste and costs to a minimum.

Features & Benefits

  • Lyophilised for enhanced stability
  • 100% human serum
  • Stable to expiry date at 2°C – 8°C
  • Reconstituted stability of up to 7 days at 2°C – 8°C
DescriptionSizeAnalytesCat No 
Apolipoprotein Calibrator 23 x 1ml4LP5047

Analytes

  • Apolipoprotein A-II
  • Apolipoprotein C-III
  • Apolipoprotein C-II
  • Apolipoprotein E

Lipoprotein (a) Calibrator

Dedicated Lipoprotein (a) calibrator specifically designed for use with the Randox Lipoprotein (a) assay.

Features & Benefits

  • Lyophilised for enhanced stability
  • 100% human serum
  • Stable to expiry date at 2°C – 8°C
  • Reconstituted stability of up to 14 days at 2°C – 8°C
DescriptionSizeAnalytesCat No 
Lipoprotein (a) Calibrator5 x 1ml1LP3404

Analytes

  • Lipoprotein (a)

Direct HDL/LDL Cholesterol Calibrator

Dedicated HDL and LDL cholesterol calibrator specifically designed for use with direct methods of measurement.

Features & Benefits

  • Lyophilised for enhanced stability
  • 100% human serum
  • Stable to expiry date at 2°C – 8°C
  • Reconstituted stability of up to 5 days at 2°C – 8°C and 1 month at -20°C
DescriptionSizeAnalytesCat No 
HDL/LDL Cholesterol Calibrator3 x 1ml2CH2673

Analytes

  • HDL Cholesterol
  • LDL Cholesterol

sdLDL Cholesterol Calibrator

Dedicated sdLDL Cholesterol calibrator designed for use with the Randox sdLDL Cholesterol assay.

Features & Benefits

  • Lyophilised for enhanced stability
  • 100% human serum
  • Stable to expiry date at 2°C – 8°C
  • Reconstituted stability of up to 5 days at 2°C – 8°C
DescriptionSizeAnalytesCat No 
sdLDL Cholesterol Calibrator3 x 1ml1CH5050

Analytes

  • sdLDL Cholesterol

Apolipoprotein Quality Control

Therapeutic Drug Quality Control

The Randox Acusera Apolipoprotein quality control is designed for use in the routine monitoring of 4 specialised Apolipoprotein assays. Assayed method specific target values and ranges are provided.

Features & Benefits

  • Lyophilised for enhanced stability
  • 100% human serum
  • Assayed target values provided for 4 specialised Apolipoproteins
  • Three levels available covering low, borderline and high risk levels
  • Stable to expiry date at 2°C – 8°C
  • Reconstituted stability of 28 days for Apolipoprotein A-II and C-III, 14 days for Apolipoprotein C-II and 8 hours for Apolipoprotein E. All analytes are stable for 4 weeks at -20°C
DescriptionSizeAnalytesCat No 
Apolipoprotein Control Level 13 x 1ml4LE5016
Apolipoprotein Control Level 23 x 1ml4LE5017
Apolipoprotein Control Level 33 x 1ml4LE5018

Analytes

  • Apolipoprotein A-1
  • Apolipoprotein C-III
  • Apolipoprotein C-II
  • Apolipoprotein E

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