Evidence Investigator: One Analyser for Multiple Food Testing Industries

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Evidence Investigator: One Analyser for Multiple Food Testing Industries

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28 September 2020

The Randox Evidence Investigator: One Analyser for Multiple Food Diagnostic Industries

The Randox Evidence Investigator has been validated for Randox Food Diagnostics across various food matrices including tissue, feed and cereals, honey, aquaculture, and milk, making it the perfect testing equipment solution for any food testing laboratory.

How can the Randox Evidence Investigator benefit me?

  • The Randox Evidence Investigator is a multi-analyte quantitative drug residue screening analyser. Using Randox’s patented Biochip Technology, the analyser ensures screening food for drug residues is accurate and efficient, offering a range of laboratories comparable results to LC-MS/MS.
  • Using multiplex technology, the Evidence Investigator can provide simultaneous detection for a wide range of analytes from a single sample, saving you time and resource, and getting the reliable results you need.
  • The analyser uses unique image processing software to translate the Relative Light Units (RLU) generated from the chemiluminescent reactions into an analyte concentration.
  • No manipulation of results is required, which reduces the scope for any operator error. The Randox Evidence Investigator provides excellent sensitivity with a quantitative concentration result (ppb) for each analyte tested.
  • The analyser boasts an extensive test menu with tests for the most widely used drug residues and the most commonly detected mycotoxins in the feed production industry.
  • When purchasing the Randox Evidence Investigator, you will receive the complete package required for sample analysis which includes the analyser, PC and imaging software, a thermoshaker and a barcode scanner.

Visit the Randox Food Diagnostics website for more information on this technology.

For all enquiries relating to food testing on any of our Randox analysers, please contact us via email at: info@randoxfooddiagnostics.com

 

Want to know more?

Contact us or visit our Randox Food Diagnostics website.

Related Products

Meat & Seafood

Milk

Honey

Wine


Familial Hypercholesterolemia

Familial Hypercholesterolemia

Rapid & Reliable Genetic Assessment

Genetic Assessment of Patients with Suspected Familial Hypercholesterolemia (FH)

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    Early diagnosis of specific mutations reducing morbidity and mortality from premature CVD ensuring better outcomes & therapeutic intervention
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    Saving time & costs by reducing the number of traditional clinical & genetic tests
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    Combination of multiplex PCR and Biochip to distinguish between multiple wildtype and mutant DNA regions
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    Cascade screening can be performed across both FH arrays to further improve diagnosis
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    Provides information on the phenotype and prognosis which cannot be identified through traditional lipid level testing
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    Suitable for use on the Evidence Investigator, a semi-automated analyser identifying FH in under 3 hours

The Familial Hypercholesterolemia (FH) Arrays I & II are rapid, simple and accurate diagnostic tests which enable simultaneous detection of 40 FH-causing mutations (20 mutations per array) within the low-density lipoprotein receptor (LDLR), apo‑lipoprotein B (ApoB) and proprotein convertase subtilisin/kexin type 9 (PCSK9) genes. Generally, mutations within the LDLR gene are the most common, with a prevalence of 1 in 500. The ApoB gene has a prevalence of 1 in 1000 and the PCSK9 gene less than 1 in 2500.

FH patients have elevated levels of total cholesterol (TC) and LDL-C from birth and, if untreated, develop coronary heart disease (CHD). FH is characterised by twice normal LDL-C levels in early childhood, and early myocardial infarction. A strong positive family history can imply as much as a 12-fold increased risk of early coronary disease.

Biochip

Randox Familial Hypercholesterolemia Arrays

  • FH Array I
  • FH Array II
ApoB
MutationProtein
c.10580G>Ap.(Arg3527Gln)
LDLR
MutationProtein
c.2292delAp.(Ile764Metfs*2)
c.1444G>Ap.(Asp482Asn)
c.551G>Ap.(Cys184Tyr)
c.1845+11C>Gp.(=)
c.693C>Ap.(Cys231*)
c.933delAp.(Glu312Serfs*58)
c.301G>Ap.(Glu101Lys)
c.313+1G>Ap.(=)
c.1706-1G>Ap.(=)
c.1706-1G>Ap.(Cys677Arg)
c.2029T>Cp.(Pro685Leu)
c.2054C>Tp.(Trp483Arg)
c.1447T>Cp.(Gly478Arg)
c.1447T>Cp.(Asp72Thrfs*134)
c.214delGp.(Trp87Gly)
c.259T>Gp.(Arg633Cys)
c.1897C>Tp.(Asp227Glu)
c.681C>Gp.(Asn688Glnfs*29)
PCSK9
MutationProtein
c.1120G>Tp.(Asp374Tyr)
LDLR
MutationProtein
c.1285G>Ap.(Val429Met)
c.680_681delACp.(Asp227Glyfs*12)
c.1187-10G>Ap.(=)
c.1048C>Tp.(Arg350*)
c.118delAp.(Ile40Serfs*166)
c.1168A>Tp.(Lys390*)
c.232C>Tp.(Arg78Cys)
c.1587-1G>Ap.(=)
c.1706-10G>Ap.(=)
c.1796T>Cp.(Leu599Ser)
c.1436T>Cp.(Leu479Pro)
c.1474G>Ap.(Asp492Asn)
c.501C>Ap.(Cys167*)
c.662A>Gp.(Asp221Giy)
c.682G>Tp.(Glu228*)
c.1150C>Tp.(Gln384*)
c.938G>Ap.(Cys313Tyr)
c.136T>Gp.(Cys46Gly)
c.2042G>Cp.(Cys681Ser)
c.1618G>Ap.(Ala540Thr)

The Evidence Investigator

Meet the Evidence Investigator

The Randox FH arrays has been developed for the Evidence Investigator, a semi-automated benchtop immunoassay analyser.

The FH arrays would provide an early diagnosis of specific mutations, reduce morbidity and mortality from premature CVD ensuring better patient outcomes & therapeutic intervention.

Evidence Investigator

Want to know more?

Contact us or visit our Investigator Webpage


Randox Alzheimer’s Array on the Evidence Investigator

Alzheimer’s disease

Alzheimer’s disease is the most common cause of dementia.  It is defined as an irreversible, progressive brain disorder, in which parts of the brain are damaged over time. As this happens symptoms develop, but also get worse.

Dr. Alois Alzheimer discovered that in Alzheimer’s disease the connections between the cells and brain tissue are lost because proteins build up and form abnormal structures called “plaques” and “tangles”. 1 A healthy brain contains important chemicals which send signals between the cells, however, those who suffer with Alzheimer’s have less “chemical messengers.” Therefore, the signals don’t get passed on. 1

Risk factors

Age is the biggest risk factor. Alzheimer’s disease is more common amongst older adults. In the UK there are over 40,000 people under the age of 65 who suffer with some form of dementia. 2 Studies also state that women over the age of 65 are twice as likely to develop Alzheimer’s disease than men – although there is no clear evidence as to why.

There are two different types of Alzheimer’s. The early on-set variant of the condition is very uncommon but strikes people younger than 65. Often people with early-onset Alzheimer’s develop symptoms in their 40s or 50s. Whereas, late-onset Alzheimers is more common and affects people age 65 and older. 2

Symptoms

The disease slowly destroys memory and thinking skills. The earliest symptoms are memory lapses where they may struggle to remember recent events or learn new information, or even forget important items for day-to-day life for example, their keys, glasses or mobile phone. Memory loss due to the disease can increasingly interfere with their life as often the ability to carry out simple tasks can become a struggle. As a result, the person suffering can become anxious, irritable and can even be depressed.

In the later stages of Alzheimer’s, the symptoms become more severe. The individual will become less aware of what’s happening around them. They may have difficulties eating, walking and will require additional help and support with their daily activities from their loved ones or from a carer.

Unfortunately, there is no cure for Alzheimer’s Disease, although, there is treatment that can help manage the symptoms.

The Randox Apolipoprotein E4 Array

Randox offers The Apolipoprotein E4 (ApoE4) Array.

The Apolipoprotein E4 (ApoE4) Array is a research use-only product developed for the Evidence Investigator, which is a semi-automated benchtop immunoassay analyser which can process up to 2376 test per hour as well as up to 44 analytes screened per biochip.

The ApoE4 Array measures both total ApoE protein levels and ApoE4 protein levels directly from plasma samples and by using a ratio it can classify patients as negative or positive for ApoE4. In turn we can then assess their risk for the development of Alzheimer’s disease.

For further information about the Randox Alzheimer’s Array or our Evidence Investigator, please email info@randoxbiosciences.com                                                                                                                                                                                                                                  

  1. https://www.alzheimers.org.uk/about-dementia/types-dementia/alzheimers-disease-symptoms
  2. https://www.nia.nih.gov/health/what-alzheimers-disease

 

 

 

 

 


The world’s most extensive Drugs of Abuse Test Menu

Randox Toxicology offers the most comprehensive Drugs of Abuse (DoA) test menu across multiple forensic matrices. Our level of expertise in toxicology research and development allows us to adapt quickly to the ever-changing market influences and develop assays for current and novel drugs trends. Excellent assay precision and performance eliminates false reporting, therefore reducing unnecessary confirmatory tests and time lost in the laboratory as a result. Our Biochip Arrays offer CVs typically less than 10%, producing an accurate drug profile to ensure confidence in results.

DoA II
Buprenorphine Ketamine Oxycodone I
Creatinine LSD Oxycodone II
Fentanyl MDMA Propoxyphene
Generic Opioids Methaqualone

The Evidence Series of immunoassay analysers are powered by Biochip Array Technology and combine the latest technological advances for drug residue detection using immunoassay principles. The Drugs of Abuse II panel is available for both the Evidence and the Evidence Investigator analysers. The Evidence has a throughput of 90 samples per hour, testing up to 44 tests per sample.  The Evidence is a fully automated batch immunoanalyser, allowing for 3960 tests per hour, while the Evidence Investigator is a semi-automated, bench top analyser with testing capabilities of 2376 tests in 70 minutes.

 

 

To find out more about our Biochip Array Technology and our Evidence Series analysers, visit www.randoxtoxicology.com, or email us at info@randoxtoxicology.com


Researching the Evidence Investigator

The Evidence Investigator analyser is based on the award-winning Biochip Array Technology (BAT). Biochip Array Technology is a multi-analyte testing platform allowing the simultaneous quantitative or qualitative detection of a wide range of analytes from a single sample.

The Evidence Investigator is a semi-automated benchtop analyser which is tailored for the areas of research, forensic, clinical, molecular and veterinary testing. The key feature is the fast turn-around time the Evidence Investigator can process up to 44 results from a single sample, with a maximum throughput of up to 2375 tests per hour. The Evidence Investigator saves time because it will carry out multiplex testing which will allow multiple tests to be carried out from a single patient sample which in return will save time and resources. The machine is more suitable for medium throughput laboratories.

The technology offers a wide-ranging and diverse test menu which will benefit the research areas immunology, metabolic, Sport & Exercise, oncology and cardiovascular.  Randox Research provides the Evidence Investigator to the five research areas to help their research become more efficient, cost effective and accurate.

Randox works extremely hard with their research and development, over 16% of turnover is reinvested in R&D. The current collaboration Randox works along with are Royal Victoria Hospital, Queen’s University Belfast and Cambridge University.

Randox Biosciences are dedicated to assisting research projects to completion and will make available the technology to ensure the universities receive highly accurate results for their research. Randox creates their products in-house therefore provides the flexibility of the research project. Randox can provide the university a full range of arrays, biomarkers and analysers to meet their requirements of the research.

Within Cardiovascular Research, Randox offer a comprehensive menu of cytokines. The combination of highly specific antibodies and advanced chemistries enables cytokines, cytokine receptors and growth factors to be detected simultaneously in a single sample, providing valuable information relating to each cytokine under test and possible associations between cytokines in each sample which will benefit the research. Randox offer excellent tools for universities and hospitals researchers such as routine and novel assays and can provide research analysers such as the Evidence Investigator which is suitable for medium sized laboratory.

Oncology Research has 20 biomarkers that can be custom-made to be used on the Biochip. Randox Biosciences offers a wide and extensive test menu to researchers to enable the specific product tailored to meet their clinical trail requirements.

Metabolic & Nutrition Research is another area Randox offers a wide-ranging range of tests specifically directed to Metabolic and Nutrition Research. Randox offers reagents and arrays on the award-winning Biochip Array Technology.

For more information on our Research areas and the tests that we can provide, contact us at – Info@RandoxBiosciences.com


Evidence Series – Investigator and Molecular testing

Evidence Investigator | Adaptable, Efficient & Comprehensive

The Evidence Investigator is a compact, semi-automated benchtop analyser that offers efficient and comprehensive testing across a range of applications including clinical diagnostics, molecular, research, toxicology and food diagnostics.

Renowned for its versatility, robustness and effective reporting methods, the Evidence Investigator has been used in a wide range of laboratory settings for over 15 years. This highly advanced yet simple to use analyser has only one moving part, giving the user peace of mind.

The Evidence Investigator contains a host of innovative on-board data analysis features ensuring manual processes are kept to a minimum.

By utilising the same multiplex technology as the other Evidence Series analysers, the Evidence Investigator can process up to 44 results from a single sample, with a maximum throughput of up to 2376 tests per hour. Offering efficiency without compromising on accuracy, the Evidence Investigator is the perfect fit for medium throughput laboratories seeking maximum use of bench space.

Molecular Testing

Our molecular product range offers diagnostic, prognostic and predictive solutions across a variety of disease areas including sexually transmitted infection (STI), Respiratory tract infection, colorectal cancer, familial hypercholesterolemia (FH) and cardiovascular disease (CVD). Additionally, we can provide a wide range of assay formats including single nucleotide polymorphisms (SNP) genotyping, pathogen detection and mutation detection.

Pathogen Detection

STI and Respiratory Arrays

Both arrays detect the most common and frequently requested infections in sexual and respiratory health. These comprehensive, highly sensitive and specific tests enable identification of co-infections simultaneously, often in asymptomatic patients and enable antibiotic stewardship.

Mutation Detection

KRAS, BRAF, PIK3CA array and Familial Hypercholesterolemia Arrays I & II

These unique biochip assays permit high discrimination between multiple targets in a number of genes with a rapid turnaround time (3 hours). The arrays enable detection of the most frequently occurring mutations known to cause disease (FH) and adversely affect patient treatment (KRAS, BRAF, PIK3CA).  A unique primer set is designed for each target which will hybridise to a complimentary oligo-nucleotide probe spotted on a biochip discrete test region (DTR).

SNP Genotyping

Cardiovascular Risk Prediction Array

This array identifies individuals with a genetic predisposition to coronary heart disease (CHD). The innovative multiplex primers are designed to discriminate DNA sequences which differ only at one base.

For more information on our Evidence Series or Molecular range of Assays, contact us at EvidenceSeries@randox.com

 

 

 


The Evidence Series

Having developed the patented Biochip Array Technology following £250 million investment in research and development, we needed a platform that allowed Biochip Array Technology capabilities to be showcased. Step forward the Evidence Series.

The series includes the Evidence, the Evidence Evolution, the Evidence Investigator and the Evidence MultiSTAT. Each analyser has been designed and built with boundary pushing engineering, to ensure financial, labour and time savings for the end user.

Have a read below of the overview of each analyser in the Evidence Series

Evidence Evolution

The world’s first fully automated random-access biochip testing platform, the Evidence Evolution is the world’s most advanced immunoanalyser. With the capability to process up to 2,640 tests per hour, the Evidence Evolution offers complete system integration, as well as the most comprehensive test menu on the market.

Evidence

As the world’s first Biochip Array Technology system, the Evidence immunoanalyser has revolutionised laboratory screening worldwide. With the capability to process 3,960 tests per hour and a sample capacity of 360, the Evidence is ideal for use in a high throughput laboratory.

Evidence Investigator

The Evidence Investigator is a compact, semi-automated benchtop immunoanalyser that offers efficient and comprehensive testing across a range of applications including clinical diagnostics, molecular, toxicology and food diagnostics. The Evidence Investigator boasts a throughput of up to 2,376 tests per hour, offering efficiency without compromising on accuracy.

Evidence MultiSTAT

The Evidence MultiSTAT is a fully automated immunoanalyser that enables on-site simultaneous detection of up to 44 analytes from a single sample of oral fluid, urine or blood. With a three-step process and results generated in less than 20 minutes, the Evidence MultiSTAT is an ideal solution for those with no knowledge of laboratory procedures and offers a throughput of up to 132 tests per hour.

 

 

 

About the Randox Evidence Series

The Evidence Series is set to revolutionise diagnostic testing forever. Offering unrivalled capabilities across all analysers, we truly believe that the Evidence Series range of immunoassay analysers can meet your diagnostic testing capabilities.

For more information on any of the Evidence Series analysers, please visit https://www.randox.com/evidence-series/ or contact us evidenceseries@randox.com.

 

 

 


Randox Biochip Array (BAT) Technology free from Biotin-Streptavidin

Biotin

Biotin, also known as vitamin B7, is involved with fatty acid metabolism, amino acid degradation, and gluconeogenesis.  The recommended daily intake for biotin is roughly 30-70µg, which is extremely low, meaning that biotin deficiency is rare.  Recently, there has been a surge in biotin supplementation mainly for beauty reasons, including: stronger nails and healthier skin and hair, resulting in the biotin craze on Instagram.  Currently 162K posts are attributed to the biotin hashtag (#biotin) on Instagram.  Whilst biotin supplementation is beneficial for numerous health conditions, including: multiple sclerosis (MS), diabetes, elevated cholesterol, and metabolic dysfunction, the increasing use of biotin by patients has created a problem with in vitro diagnostic testing.

With numerous manufacturers using biotin-streptavidin technology to develop in vitro diagnostic tests, combined with the rise in biotin supplementation use, the FDA (food and drug administration) issued an alert regarding the potential for erroneous results triggered by high levels of biotin in patient samples, at the end of 2017.  Clinical decisions based on these false results from biotin technology can lead to inaccurate diagnosis and inappropriate treatment prescribed.  The FDA confirmed that a patient, who was consuming high levels of biotin, died when a troponin tested was skewed and failed to show that the patient was having a heart attack.  Other tests that can produce erroneous results include: cardiac, pregnancy, cancer and iron-deficiency tests.

Biotin-Streptavidin

(Halasey, 2018)

The image above highlights that in biotin-free patient samples, the analyte to be tested successfully binds to the biotinylated antibody and the labelled antibody ultimately ensuring accurate measurement.  In the patient sample containing high levels of biotin, the biotin inhibits streptavidin’s ability to capture the analyte-antibody complex, generating falsely lowered results.

As 70% of all clinical decisions are based on results from in vitro diagnostic tests, it is vital that laboratories are selecting in vitro diagnostic tests that do not adopt the biotin-streptavidin technology to ensure accurate patient testing.

Randox do not utilise the biotin-streptavidin technology in the development of the Biochip Array Technology (BAT).

The Randox Biochip facilitates multiplex testing for faster, more comprehensive patient testing.  This technology, free from biotin-streptavidin, uses capture antibodies bound to a solid substrate (biochip surface) as opposed to biotinylated antibodies used by other manufacturers.  The Biochip also utilises enzyme labelled conjugate to enable chemiluminescent detection of the target in the patient sample.

Biochip test menu

The Biochip Array Technology (BAT) from Randox is capable of simultaneous multi-analyte diagnostic testing within the fields of clinical research and drugs of abuse testing.  The technology works through combining a panel of related assays on a single biochip with a single set of reagents, controls and calibrators.  An extensive range of Biochip panels are available, each optimised to provide the best performance.

Evidence

Evidence

The evidence analyser is the world’s first protein Biochip Array Technology system and has truly transformed laboratory diagnostics worldwide.  As the first of its kind, the Evidence has introduced higher standards of quality efficiency and reliability to numerous sectors including hospitals and clinical laboratories, forensic and clinical toxicology, pharmaceutical/CRO applications, as well as veterinary laboratories.

Evidence Evolution

Evolution

The world’s first fully automated random access Biochip testing platform.  The Evidence Evolution is set to revolutionise current diagnostic testing.  With the capability to process up to 2,640 tests per hour, the Evidence Evolution utilises multiplexing technology, offering advanced test consolidation, patient profiling, a complete system integration, as well as the most comprehensive test menu on the market.

Investigator

Investigator

The #1 choice for research, clinical, forensic, and veterinary testing.  Using the multiplexing technology, the semi-automated benchtop immunoanalyser Evidence Investigator is suitable for medium throughput laboratories.  In addition to the current test menu for this analyser, Randox have new tests in development.

Reference:

Halasey. S, 2018, “Inside Track: Biotin Gets a Safety Alert”. Available from URL: http://www.clpmag.com/2018/01/inside-track-biotin-gets-safety-alert/


Evidence Investigator | Adaptable, Efficient & Comprehensive

The Evidence Investigator is a compact, semi-automated benchtop analyser that offers efficient and comprehensive testing across a range of applications including clinical diagnostics, molecular, research, toxicology and food diagnostics.

Renowned for its versatility, robustness and effective reporting methods, the Evidence Investigator has been used in a wide range of laboratory settings for over 15 years. This highly advanced yet simple to use analyser has only one moving part, giving the user peace of mind.

The Evidence Investigator contains a host of innovative on-board data analysis features ensuring manual processes are kept to a minimum.

By utilising the same multiplex technology as the other Evidence Series analysers, the Evidence Investigator can process up to 44 results from a single sample, with a maximum throughput of up to 2376 tests per hour. Offering efficiency without compromising on accuracy, the Evidence Investigator is the perfect fit for medium throughput laboratories seeking maximum use of bench space.

 

Accurate and Robust

Like all the Evidence Series analysers, what sets it apart is technology. The Evidence Investigator is extremely well equipped to provide reliable results, while simultaneously robust enough to withstand frequent, heavy use.

Results are generated using a Charge Coupled Device (CCD) camera, which quantifies chemiluminescent light. This light measures the degree of binding between the patient sample and specific biochip bound ligands, generating highly accurate and reliable results.

Consolidation

The Evidence Investigator is the world’s first platform allowing consolidation of immunoassay and molecular diagnostics. This is achieved through utilising protein and DNA based biochips. By giving the user the ability to consolidate tests, the Evidence Investigator improves laboratory efficiency and reduces costs.

Advanced Reporting

The Evidence Investigator image processing software translates light signal generated from chemiluminescent reactions into analyte concentration. This removes the need for any manual processing of data.

Previously unreported tests can also be retrieved, so they can be tested retrospectively. This saves time, labour costs and reduces any reagents wastage. All data is then analysed on-board, removing issues related to human error and result manipulation.

About the Randox Evidence Series

The Evidence Series is set to revolutionise diagnostic testing forever. Offering unrivalled capabilities across all analysers, we truly believe that the Evidence Series range of immunoassay analysers can meet your diagnostic testing capabilities.

For more information on the Evidence Investigator, or any of the Evidence Series analysers, visit https://www.randox.com/evidence-series/ or contact us evidenceseries@randox.com.

 

 

 

 

 

 

 


Biochip Carrier Applications in the Randox Evidence Series

Biochip Array Technology is the fundamental component which drives the Randox Evidence Series immunoanalysers. The simultaneous detection of multiple analytes from a single sample allows for a complete patient profile, providing exceptional time, resource and cost savings.

The Evidence Series analysers all carry the Biochip in different applications. In order to gain a better understanding of how it is utilised, the illustrations below demonstrate how adaptable and flexible the technology is across the Evidence Series.

Evidence Evolution

  • One strip contains 10 biochips
  • Analyser can hold 20 strips
  • Can produce a throughput of up to 2640 tests per hour

Evidence

  • Each cassette contains 10 carriers
  • Each carrier has 9 wells
  • Analyser can hold 4 cassettes at once
  • Can produce a throughput of up to 3960 tests per hour

 Evidence Investigator

  • Analyser holds 1 cartridge
  • 1 cartridge contains 9 wells
  • Thermoshaker capable of carrying 6 cartridges
  • Can produce a throughput of up to 2376 tests per hour

 Evidence MultiSTAT

  • Analyser holds 1 cartridge
  • Cartridge comes pre-loaded with reagents and QC
  • Can produce a throughput of up to 132 tests per hour

 

About the Randox Evidence Series

The Evidence Series is set to revolutionise diagnostic testing forever. Offering unrivalled capabilities across all analysers, we truly believe that the Evidence Series range of immunoassay analysers can meet your diagnostic testing capabilities.

For more information on any of the Evidence Series analysers, visit https://www.randox.com/evidence-series/ or contact us evidenceseries@randox.com.

 

 

 


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